Animal Hypoxia Core

动物缺氧（核心C）的主要目的是提供标准化的 持续和间歇性低氧和/或高碳酸血症的苍蝇和小鼠使用的所有项目， 表征不同物种、基因型中的基本呼吸和心血管表型， 个别项目研究的风险。标准化曝光对于整合调查结果至关重要 在不同的项目之间，优化数据共享。将其集中起来还可以确保质量控制， 传播单个项目的任何结果，建议应 调整重要的是，这种集中化还保护了动物和资源，以最大限度地提高预算， 动物献祭行为测量不包括在这个修订后的建议，但更大的生物圈 将使用容纳多只动物和笼子的室进行所有慢性暴露，以确保 社会互动和条件反射在核心中对小鼠进行表型分析还可以最大限度地降低成本， 个体动物模型在基本代谢、呼吸和心血管方面的信息 生理学，这是必要的所有个别项目。鉴于维持疾病的挑战 对于多系转基因小鼠的自由繁殖菌落， 在分发之前，在生理学核心设施内进行非侵入性和非终端描述性测量 将长期接触的动物送到各个实验室进行实际实验。生理数据将 上传到系统生物学核心B开发的数据库，供所有研究人员使用， 项目可以集中在生物学上。用于动物表型的生理技术培训 也将由核心C提供给在个人工作的博士前学生和博士后研究员 项目

The primary objective of the Animal Hypoxia (Core C) is to provide standardized chronic exposures of sustained and intermittent hypoxia and/or hypercapnia to flies and mice used by all of the Projects, and to characterize the essential respiratory and cardiovascular phenotypes in the different species, genotypes, and exposures studied by the individual Projects. Standardizing exposure is vital for integrating the findings between the different Projects and optimizing data sharing. Centralizing this also insures quality control and dissemination of any results from individual Projects suggesting that the exposure protocols should be adjusted. Importantly, this centralization also conserves animals and resources to maximize the budget and animal sacrifice. Behavioral measurements are not included in this revised proposal but the larger Biospherix chambers, which house multiple animals and cages will be used for all chronic exposures to insure identical social interactions and conditioning. Phenotyping mice in the core also minimizes the cost and maximizes the information individual animal models in terms of the basic metabolic, respiratory and cardiovascular physiology that is necessary for all of the individual Projects. Given the challenges of maintaining disease free-breeding colonies for multiple lines of transgenic mice, it is most efficient and cost effective to make non-invasive and non-terminal descriptive measurements within a physiology core facility before distributing chronically exposed animals to individual laboratories for the actual experiments. Physiological data will be uploaded to a database developed with Systems Biology Core B for all the investigators to use so the projects can focus on the biology. Training in the physiological techniques used to phenotype the animals will also be provided by Core C to predoctoral students and postdoctoral fellows working in the individual Projects.