Compact X-ray Station for Protein Crystallography

描述（由申请人提供）：在这份快速通道SBIR的竞争性资助修订版中：“用于蛋白质晶体学（CXS）的紧凑型X射线站”，我们描述了对紧凑型光源（CLS）的拟议增强和升级，这将导致CXS的X射线通量大幅增加。CXS是一个设计和构建X射线光学器件、终端站和软件的组合的项目，这些X射线光学器件、终端站和软件使用CLS产生的单色可调X射线束。CXS项目虽然尚未完成，但已经为学术和企业研究中心的结构生物学家提供了一种几乎交钥匙的科学仪器--一种类似同步加速器的X射线系统。为了显著减少数据收集时间，特别是对于小晶体，需要增强CLS。为了响应NIH通知：“NIH宣布恢复法案资金可用于竞争性修订申请”- NOT-OD-09-058，我们计划扩大CXS资助的范围，以包括CLS的几个硬件升级，并为额外的操作时间提供资金。这些努力将使CLS的X射线通量显著增加，可靠性更高，这将大大提高CXS的影响和性能。 公共卫生相关性：蛋白质晶体学紧凑型X射线站（CXS）与紧凑型光源（CLS）一起沿着，提供了一种技术，可为当地或区域实验室的结构生物学家带来最先进的大分子晶体学。该工具将允许更广泛的研究人员进行广泛的结构测定实验，从而更好地了解蛋白质和其他大分子聚集体在疾病和治疗中的作用。

DESCRIPTION (provided by applicant): In this Competitive Grant Revision to the Fast-Track SBIR: "A Compact X-ray Station for Protein Crystallography (CXS)," we describe proposed enhancements and upgrades to the Compact Light Source (CLS) that will result in a substantial increase in x-ray flux to the CXS. The CXS is a project to design and build a combination of x-ray optics, endstations, and software that use the monochromatic, tunable x-ray beam produced by the CLS. The CXS project, though not yet complete, has already resulted in an almost turnkey scientific instrument - a synchrotron-like x-ray system - for structural biologists at academic and corporate research centers. In order to make a significant reduction in data collection time, especially for small crystals, enhancements to the CLS are needed. In response to the NIH Notice: "NIH Announces the Availability of Recovery Act Funds for Competitive Revision Applications" - NOT-OD-09-058, we plan to expand the scope of the CXS grant to include several hardware upgrades to the CLS and to fund additional operations time. These efforts will result in significantly more x-ray flux and better reliability of the CLS, which together will greatly improve the impact and performance of the CXS. PUBLIC HEALTH RELEVANCE: The Compact X-ray Station for Protein Crystallography (CXS), along with the Compact Light Source (CLS), provides the technology to bring state-of-the-art macromolecular crystallography to structural biologists in their own local or regional laboratories. This tool will allow a wider community of researchers to conduct a broad range of structure determination experiments that will lead to better understanding of the role of proteins and other macromolecular assemblages involved in disease and therapy.