A Biological Small Angle X-ray Scattering Station (BioSAXS) for the Compact Light

紧凑型光源生物小角X射线散射站(BioSAXS)

• 批准号: 8648498
• 负责人: RODERICK J LOEWEN
• 金额: $ 18.94万
• 依托单位: LYNCEAN TECHNOLOGIES, INC.
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-07-01 至 2016-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8648498
• 关键词: Architecture; Benchmarking; Biological; Biological Process; Cells; Collimator; Complement; Complex; Crystallography; Data; Disease; Elements; Engineering; Environment; Equipment; Future; Generations; Geometry; Goals; Grant; Image; Laboratories; Laboratory Research; Light; Macromolecular Complexes; Measurement; Mechanics; Methods; Modeling; Molecular; Molecular Structure; Motion; Noise; Optics; Output; Performance; Pharmaceutical Preparations; Phase; Play; Proteins; Renaissance; Research; Resolution; Roentgen Rays; Role; Sampling; Shapes; Signal Transduction; Small Business Innovation Research Grant; Solutions; Source; Specific qualifier value; Spottings; Staging; Structure; Synchrotrons; System; Techniques; Technology; Testing; Work; X-Ray Crystallography; base; beamline; design; detector; experience; frontier; instrument; macromolecule; protein structure; prototype; public health relevance; research study; structural biology; success; tool

7. Project Summary/Abstract We propose to develop a Biological Small-angle X-ray Scattering (BioSAXS) station for the Compact Light Source, a powerful new generation of X-ray sources, which will enable biologists and biochemists to pursue state-of-the-art research in macromolecular structure studies in their own laboratories. The frontier of structural biology is the determination of large macromolecular complexes-the machines that drive the workings of the cell. X-ray crystallography is the method of choice for revealing the structures of these complexes, and has had important successes. However, since many complexes have proven to be difficult or impossible to crystallize, alternative methods are required. Lower resolution methods like SAXS can provide overall shape information of the complex that can be used, together with known substructures, for modeling its architecture. The advent of synchrotron sources together with advanced computing has led to a renaissance for SAXS as a complementary technique to crystallography for understanding a variety of molecular complexes. By developing a BioSAXS beamline and endstation, the CLS will offer biologists a new tool to perform synchrotron-like quality SAXS in their own academic or research laboratories at resolutions currently unreachable using conventional source technology. The flux of this BioSAXS beamline will exceed presently available homelab SAXS beamlines by three orders of magnitude, providing results comparable to many productive SAXS beamlines at the synchrotrons. The BioSAXS beamline is expected to complement the existing crystallography endstation already developed for the CLS to provide a unique, combined product for leading-edge biophysical analysis.

7.项目总结/摘要 我们建议开发一个生物小角X射线散射（BioSAXS）站的紧凑光 源，强大的新一代X射线源，这将使生物学家和生物化学家， 在自己的实验室里进行高分子结构研究的最新研究。边疆 结构生物学的一个重要方面是确定大分子复合物--驱动细胞生长的机器。 细胞的运作。X射线晶体学是揭示这些晶体结构的首选方法。 复杂，并取得了重要的成功。然而，由于许多复合物已被证明是 结晶困难或不可能，需要替代方法。SAXS等较低分辨率方法 可以提供可以使用的复合体的整体形状信息，连同已知的子结构， 来模拟它的结构。同步辐射源的出现以及先进的计算技术 导致了SAXS作为晶体学补充技术的复兴， 各种分子复合物。通过开发BioSAXS光束线和终端站，CLS将提供 生物学家在他们自己的学术或研究中执行同步加速器质量SAXS的新工具 实验室的分辨率目前无法达到使用传统的源技术。这种流动 BioSAXS光束线将超过目前可用的家庭实验室SAXS光束线三个数量级， 的幅度，提供的结果相媲美的许多生产SAXS光束线在同步加速器。的 BioSAXS光束线预计将补充现有的晶体学终端站已经开发 为CLS提供一个独特的，结合产品的前沿生物物理分析。