MOLECULAR CHARACTERIZATION OF THE BOCAVIRUSES

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者的研究机构。 背景资料：博卡病毒是细小病毒亚科的一个属，包括牛细小病毒（BPV）、犬细小病毒（CnMV）和最近鉴定的人博卡病毒（HBoV）。HBoV与儿童的下呼吸道感染有关，并已被认为占全球下呼吸道感染的5%。 基本原理：目前，HBoV的病毒和感染性DNA都不可用，这些动物博卡病毒的表征最终将为博卡病毒属提供一般信息。 研究问题：了解人类HBoV感染的流行病学以及博卡病毒如何在宿主细胞中复制的分子基础。 结局指标：建立检测血清中抗HBoV抗体的血清学方法，构建博卡病毒感染性克隆，研究博卡病毒蛋白在病毒复制中的作用。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Background: Bocavirus is a genus of subfamily Parvovirinae, which includes bovine parvovirus (BPV), canine minute virus (CnMV) and the recently identified human bocavirus (HBoV). HBoV has been associated with lower respiratory tract infections in children, and has been suggested to account for as much as 5% of lower respiratory tract infections worldwide. Rationale: Currently, both virus and infectious DNA of HBoV are not available, characterization of these animal bocaviruses will eventually provide information to the genus Bocavirus in general. Study question: Understanding the epidemiology of HBoV infection in humans and the molecular basis how bocaviruses replicate in host cells. Outcome measures: Establish a serological method to detect antibodies against HBoV in sera and construct infectious clones of bocaviruses to dissect the roles of bocavirus proteins in viral replication.