COBRE: WVU: SIGNAL TRANSDUCTION & CANCER: MASS SPECTROMETRY & PROTEOMICS CORE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 西弗吉尼亚大学蛋白质组学核心设施成立于2001年，以前提供的服务包括MALDI-TOF-MS和LC-MS/MS蛋白质鉴定以及二维凝胶蛋白质分离和分析。该核心被设计用于五个功能：1)差异表达分析或识别和相对定量两个不同样本之间上调和下调的蛋白质，2)功能蛋白质分析，3)蛋白质识别，4)微观表征，以识别和绘制蛋白质的翻译后修饰，以及5)结构分析。这一核心将于2009年6月30日关闭，原因是使用率不佳，使其不再可行。核心用途没有显示出对其设计所针对的服务的一致或高需求。这可能是由于近年来出现的技术变化，或者是以更及时和更具成本效益的方式提供相同服务的商业来源的增加。因此，根据内部顾问委员会的建议，资金将重新用于我们的成像和蛋白质核心设施，这些设施包括Cobre研究人员以及HSC其他科学家高度寻求的功能。本财年的记录将成像核心使用量排在HSC最高之列，其中Cobre相关调查团队的使用量很大。一项对蛋白质核心的前瞻性调查显示，需求很高，所需的Cobre支持相对较少。这些核心将由Karen Martin博士和Stephen Graeber博士监督，如09-10财政年度预算所示

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The WVU Proteomics Core Facility was established in 2001, and previously offered services including MALDI-TOF-MS and LC-MS/MS protein identification and 2-D gel protein separations and analysis. This core was designed to serve five functions: 1) Differential expression analysis or the identification and relative quantification of up- and down-regulated proteins between two different samples, 2) Functional protein analysis, 3) Protein identification, 4) Micro-characterization, to identify and map post-translational modifications of proteins, and 5) Structural analysis. This core will be shut down June 30, 2009 based on sub-optimal usage making it no longer viable. The core use did not demonstrate a consistent or high demand for the services for which it was designed. This may be due to changes in technology that have emerged in recent years, or increased availability of commercial sources that provide the same services in a more timely and cost efficient manner. As such, based on the recommendation of the internal advisory board, funds will be re-directed into our imaging and protein core facilities that include functions highly sought out by both COBRE investigators as well as other scientists in the HSC. Records from the current fiscal year rank imaging core use among the highest at the HSC, with significant use by COBRE associated investigative teams. A prospective survey of the protein core, indicates high demand, with the COBRE support required being relatively modest. These cores will be overseen by Drs. Karen Martin and Stephen Graeber as indicated in the budget for fiscal year 09-10