COBRE: WVU: SIGNAL TRANSDUCTION & CANCER: MASS SPECTROMETRY & PROTEOMICS CORE

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 中心，不一定是研究者的机构。 WVU蛋白质组学核心设施成立于2001年，是COBRE赠款的一部分，目前正在为WVU和邻近机构提供MALDI-TOF-MS和LC-MS/MS蛋白质鉴定和2-D凝胶蛋白质分离和分析服务。该核心将提供五个功能：1）差异表达分析或两个不同样品（健康与患病）之间上调和下调蛋白质的鉴定和相对定量。2)功能蛋白分析，将分离和鉴定复合物中相关的蛋白质。3)蛋白质鉴定，将通过凝胶电泳、蛋白质印迹或色谱法鉴定分离的蛋白质。4)微表征，将识别和映射蛋白质的翻译后修饰。5)结构分析，它将使用距离约束来确定蛋白质的三维结构。这一最终能力涉及在特定官能团处交联蛋白质、蛋白水解消化和MS分析。交联肽的MS分析提供了关于这些肽在三维结构中的接近度的信息。在第一个功能中可以是确定细胞、组织或生物流体的蛋白质组补体。此续订申请描述：1）该设施的历史，目前的仪器和人员，2）核心设施运营计划，3）在实现COBRE资助的研究目标和其他研究计划在西弗吉尼亚大学的核心设施的作用，4）在“信号转导和癌症”的研究项目，将在COBRE赠款的下一个资金周期的核心设施支持，（5）为该基金争取更多资金的努力取得进展。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The WVU Proteomics Core Facility was established in 2001 as part of the COBRE grant, and is currently offering MALDI-TOF-MS and LC-MS/MS protein identification and 2-D gel protein separations and analysis as a service to WVU and neighboring institutions. This core will serve five functions: 1) Differential expression analysis or the identification and relative quantification of up- and down-regulated proteins between two different samples (health versus diseased). 2) Functional protein analysis, which will isolate and identify proteins associated in complexes. 3) Protein identification, which will identify proteins isolated by gel electrophoresis, western blotting or chromatography. 4) Micro-characterization, which will identify and map post-translational modifications of proteins. 5) Structural analysis, which will use the distance constraints for determination of a protein's three-dimensional structure. This final capability involves cross-linking proteins at specific functional groups, proteolytic digestion, and MS analysis. MS analysis of cross-linked peptides provides information on proximity of these peptides in three-dimensional structure. Embodied in the first function can be the determination of the proteomic complement of a cell, tissue or biological fluid. This renewal application describes: 1) the Facility's history, current instrumentation, and personnel, 2) the Core Facility Operational Plan, 3) the role of the Core Facility in fulfilling research objectives in COBRE-funded and other research programs at WVU, 4) research projects in 'Signal Transduction and Cancer' that will be supported by the Core Facility in the next funding cycle of the COBRE grant, and 5) progress on efforts to obtain additional funding for the Facility.