COBRE: WVU: SIGNAL TRANSDUCTION & CANCER: MASS SPECTROMETRY & PROTEOMICS CORE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 西弗吉尼亚大学蛋白质组学核心基金成立于2001年，是科布雷赠款的一部分，目前正在向西弗吉尼亚大学和邻近机构提供MALDI-TOF-MS和LC-MS/MS蛋白质鉴定和二维凝胶蛋白质分离和分析服务。这一核心将提供五项功能：1)差异表达分析，或识别和相对量化两个不同样本(健康和疾病)之间上调和下调的蛋白质。2)功能蛋白质分析，它将分离和鉴定与复合体相关的蛋白质。3)蛋白质鉴定，用于鉴定通过凝胶电泳法、蛋白质印迹法或层析法分离得到的蛋白质。4)微观表征，它将识别和绘制蛋白质翻译后修饰的图谱。5)结构分析，它将使用距离约束来确定蛋白质的三维结构。这种最终的能力包括特定功能基团的交联蛋白、蛋白水解酶和MS分析。交联肽的MS分析提供了这些多肽在三维结构中的接近程度的信息。体现在第一个功能中的可以是确定细胞、组织或生物液的蛋白质组补体。这份续签申请描述了：1)该机构的历史、目前的仪器和人员，2)核心机构的运作计划，3)核心机构在实现Cobre资助的研究目标和西弗吉尼亚大学其他研究项目中的作用，4)将在Cobre赠款的下一个资助周期中由核心机构支持的“信号转导和癌症”研究项目，以及5)为该机构获得额外资金的努力的进展。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The WVU Proteomics Core Facility was established in 2001 as part of the COBRE grant, and is currently offering MALDI-TOF-MS and LC-MS/MS protein identification and 2-D gel protein separations and analysis as a service to WVU and neighboring institutions. This core will serve five functions: 1) Differential expression analysis or the identification and relative quantification of up- and down-regulated proteins between two different samples (health versus diseased). 2) Functional protein analysis, which will isolate and identify proteins associated in complexes. 3) Protein identification, which will identify proteins isolated by gel electrophoresis, western blotting or chromatography. 4) Micro-characterization, which will identify and map post-translational modifications of proteins. 5) Structural analysis, which will use the distance constraints for determination of a protein's three-dimensional structure. This final capability involves cross-linking proteins at specific functional groups, proteolytic digestion, and MS analysis. MS analysis of cross-linked peptides provides information on proximity of these peptides in three-dimensional structure. Embodied in the first function can be the determination of the proteomic complement of a cell, tissue or biological fluid. This renewal application describes: 1) the Facility's history, current instrumentation, and personnel, 2) the Core Facility Operational Plan, 3) the role of the Core Facility in fulfilling research objectives in COBRE-funded and other research programs at WVU, 4) research projects in 'Signal Transduction and Cancer' that will be supported by the Core Facility in the next funding cycle of the COBRE grant, and 5) progress on efforts to obtain additional funding for the Facility.