PR COMPREHENSIVE CTR FOR HIV DISPARITIES: PSM: LAB CORE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 具体目标： 核心实验室活动的具体目标几乎保持不变。此外，该实验室提交了一份提案，建议成为新的RCMI临床和转化研究(RCTR)方案下实验室核心的一部分，该方案的支持是通过波多黎各大学医学院校区(圣胡安)、中央加勒比大学(UCC；巴亚蒙)和庞塞医学院(PSM，庞塞)的联合体申请的。这一实验室核心将为参加该中心积极资助或新兴项目的患者提供几乎相同类型的艾滋病毒相关检测临床评估。对用户群体进行关于正在提供的测试的效用的教育将是新增加的优先领域之一。以下测试是通过核心实现的。 1.艾滋病病毒和丙型肝炎病毒的酶联免疫吸附试验和免疫印迹分析。 2.流式细胞术分析CD4T细胞计数、T细胞亚群产生的细胞内细胞因子和细胞外产生的细胞因子-趋化因子的定量。 3.用罗氏Amplicor程序评估HIV和丙型肝炎病毒载量。 4.线性探针法(LIPA)进行丙型肝炎病毒分型。 5.用西门子Trugene和内部程序进行HIV耐药突变分析(基因分型)。自2008年以来，这两种程序都由VQA进行了两年一次的熟练程度测试，并得到了世卫组织艾滋病毒研究网络的认可。 6.艾滋病毒共受体需求分析(CCR5与CXCR4)。 7.HIV感染T细胞的流式细胞术分析。 8.用罗氏HPV DNA芯片进行HPV基因分型。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. SPECIFIC AIMS: The specific aims of the core laboratory activity remain virtually unchanged. In addition, the laboratory submitted a proposal to be a part of laboratory core under the new RCMI Clinical and Translational Research (RCTR) program, support for which was applied through the consortium of the University of Puerto Rico Medical Sciences campus (San Juan), Universidad Central del Caribe (UCC; Bayamon) and Ponce School of Medicine (PSM, Ponce). This laboratory core will provide virtually the same types of HIV related testing for clinical evaluation of patients enrolled in the center's actively funded or emerging projects. Education of the user population on the utility of tests being provided will be one of the newly added priority areas. The following tests are implemented through the core. 1. HIV and HCV ELISA and HIV Western blot analyses. 2. Flow cytometric analysis of CD4 T cell enumeration, intracytoplastic cytokine production by T cell subsets and quantification of extra-cellularly produced cytokines-chemokines. 3. HIV and HCV viral load assessment by Roche Amplicor procedures. 4. HCV Genotyping by the line-probe assay (LIPA). 5. HIV drug resistance mutation analysis (genotyping) by the Siemens TruGene and in-house procedures. Both procedures have been biannually proficiency tested by VQA and accredited by the WHO HIV ResNet since 2008. 6. HIV coreceptor requirement analysis (CCR5 vs. CXCR4). 7. Flow cytometric analysis of HIV infected T cells. 8. Human papillomavirus (HPV) genotyping by the Roche HPV DNA Array.