T REGULATORY CELLS IN AFRICAN GREEN MONKEYS AND PIGTAILED MACAQUES

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 前言：在SIV感染后的猕猴中观察到慢性免疫激活和进展为艾滋病，而在其他灵长类物种中没有观察到。 方法：为了更好地理解这种二分法，我们利用流式细胞仪和微阵列技术，比较了非洲绿猴在急性病理性SIV感染过程中的表型标志物、免疫激活水平和CD4T细胞亚群(如Th17和Treg细胞)的耗竭程度。 结果和讨论：感染SIVagm的PTM，而不是感染SIVagm的AGM，迅速发展为全身免疫激活，显著和选择性地耗尽分泌IL-17的TH17细胞，并在血液、LNS和粘膜组织中失去Th17和T调节细胞之间的平衡。Th17细胞的丢失被发现预示着系统性和持续性的T细胞激活。总而言之，这些数据表明Th17/Treg平衡的丧失与SIV疾病的进展有关。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Introduction: Chronic immune activation and progression to AIDS are observed after SIV infection in macaques but not in other primate species. Methods: To better understand this dichotomy, we compared phenotypic markers, levels of immune activation and degree of depletion of subsets of CD4 T cells such as Th17 and Treg cells during acute pathogenic SIV infection in pigtailed macaques (PTMs) to nonpathogenic infection in African green monkeys, by flow-cytometry and microarrays. Results and Discussion: SIVagm-infected PTMs, but not SIVagm-infected AGMs, rapidily developed systemic immune activation, marked and selective depletion of IL-17-secreting TH17 cells and loss of the balance between Th17 and T regulatory cells in blood, LNs and mucosal tissues. The loss of Th17 cells was found to be predictive of systemic and sustained T cell activation. Collectively, these data indicate that loss of the Th17 to Treg balance is related to SIV disease progression.