Clinical Specimen, Data Collection, and Expression Array Core

该计划项目中的四个项目都有一个共同的主题，那就是开发新的CELL方法 和基因治疗，并使用斑马鱼模型来加速改善对 肌肉营养不良症。项目PI有着悠久而广泛的互动历史，并具有 多年来交换了许多想法、活组织检查样本和其他试剂。该计划项目进一步 加强这些协作，以产生超出任何一项范围的协同结果 实验室。临床标本、数据收集和表达数组核心(核心B)的服务包括 计划项目实现这些目标的重要手段。核心B允许增加 提高效率，并为每个项目和整个计划的数据分析提供标准化。 为此，核心B的目标旨在最大限度地提高效率并最大限度地减少行政管理 以及技术努力和费用。目标1.开展招募参与者所需的一切活动 计划项目，包括以下内容：a)确定并登记计划项目的参与者；b) 从参与者那里获得全面的数据和样本，包括临床信息、血液样本、 和肌肉组织样本；c)获得对照组织和样本；d)编目和跟踪临床和 数据库中的诊断数据；以及e)维护HNDP网站(所服务的项目：1、2、3、4、核心C)。目标 2.为计划项目进行基因表达阵列分析：a)制备斑马鱼胚胎和 全鱼、细胞和肌肉样本用于基因表达分析、分离mRNA并合成标记 用于与Affymetrix基因芯片阵列和/或Illumina Sentrix珠芯片杂交的cDNA/cRNA；b) 与Affymetrix基因芯片阵列和/或Illumina Sentrix珠芯片杂交；c)转移 将基因表达数据存储到HNDP数据库ProjectManager中，以便在Core C中进行分析。 核心C)。核心B将与核心C广泛互动，在那里，对基因表达的大部分分析 数据将会发生。

The four projects in this Program Project share the common theme of developing newer approaches to cell and gene therapy, and using zebrafish models to accelerate improvements in understanding and treating the muscular dystrophies. The Program Pi'shave a long and extensive history of interaction and have exchanged many ideas, biopsy samples, and other reagents over many years. This Program Project further strengthens these collaborations to produce synergistic results that are beyond the scope of any one laboratory. The services of the Clinical Specimen, Data Collection, and Expression Array Core (Core B) are an important means by which the Program Project will meet these goals. Core B allows for increased efficiency and provides standardization to the analysis of data for each Project and the Program overall. Toward this end, the Aims of Core B are designed to maximize efficiency and minimize both administrative and technical effort and expense. Aim 1. To carry out all activities necessary for participant recruitment into the Program Projects, including the following: a) identify and enroll participants for the Program Project; b) acquire comprehensive data and specimens from participants, including clinical information, blood samples, and muscle tissue samples; c) acquire control tissues and specimens; d) catalogue and track the clinical and diagnostic data in a database; and e) maintain the HNDP web site (Projects served: 1, 2, 3, 4, Core C). Aim 2. To perform gene expression array analysis for the Program Project: a) Prepare zebrafish embryos and whole fish, cells, and muscle samples for gene expression analysis, isolate mRNA, and synthesize labeled cDNA/cRNA for hybridization to the Affymetrix GeneChip array and/or the Illumina Sentrix BeadChip; b) Hybridize cDNA to the Affymetrix GeneChip array and/or the Illumina Sentrix BeadChip; and c) Transfer gene expression data to ProjectManager, a HNDP database, for analysis in Core C. (Projects served: 1, 2, 3, Core C). Core B will interact extensively with Core C, where much of the analysis of the gene expression data will take place.