The application of clinical microbiological methods to the study of MRSA in the environment (discipline hop)

MRSA等病原菌以及抗生素耐药菌普遍增加的患病率正在英国人群中引起显著的发病率和死亡率。迄今为止，大多数研究工作集中在临床环境中的耐药性研究，因为这是感染明显的地方。然而，越来越清楚的是，许多人类病原体，除了经典的食源性物种，如沙门氏菌，存在于环境中，特别是在农场动物种群。这些环境水库传统上由微生物生态学家研究，本培训计划的目的是让微生物生态学家Will Gaze博士接受临床微生物学理论和方法的培训。Gaze博士将在Peter Hawkey教授（Heartlands医院临床顾问微生物学家，西米德兰兹地区微生物学家，伯明翰大学临床微生物学教授）的实验室工作。Gaze博士将接受技术方面的强化培训，包括鉴定、药敏试验、分型（PFGE、MLST、SIRU）和IDI实时检测。培训将针对临床MRSA分离株的分析，但也将包括动物分离株和分子分析方法在Gaze博士提供的总社区DNA样本中的应用。MRSA等病原体的跨学科研究对于充分了解其流行病学和环境水库的程度是必要的，拟议的学科跳跃将使Gaze博士能够在未来制定和进行此类研究。

Pathogenic bacteria such as MRSA, and the increased prevalence of antibiotic resistant bacteria in general, are causing significant morbidity and mortality within the UK human population. To date the majority of research effort has concentrated on the study of resistance within the clinical setting as this is where infections are evident. However, it is becoming clear that many human pathogens, in addition to the classical food-borne species such as Salmonella, are present in the environment and particularly in farm animal populations. These environmental reservoirs have traditionally been studied by microbial ecologists, and it is the aim of this training proposal to allow Dr. Will Gaze, a microbial ecologist, to receive training in clinical microbiological theory and method. Dr Gaze will work in the laboratories of Prof. Peter Hawkey (clinical consultant microbiologist at Heartlands Hospital, Regional Microbiologist for the West Midlands, Professor of Clinical Microbiology at Birmingham University). Dr. Gaze will receive intensive training in techniques including, identification, susceptibility testing, typing (PFGE, MLST, SIRUs) and IDI real-time detection. Training will be targeted to analysis of clinical MRSA isolates, but will also include animal isolates and application of molecular analytical methods in total community DNA samples provided by Dr. Gaze. Interdisciplinary studies of pathogens such as MRSA are necessary to fully understand their epidemiology and the extent of environmental reservoirs, the proposed discipline hop will leave Dr. Gaze well placed to formulate and conduct such research in the future.