IN VITRO STUDIES OF HIV IN PRIMARY CULTURES OF HUMAN BLOOD CELLS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 研究Nef对原代培养的人细胞蛋白质转运和病毒感染性的影响。NEF是HIV-1毒力的重要贡献者。在连续的人类细胞系中，与NEF表达相关的各种表型，包括它下调细胞表面的CD4和I类MHC的能力，以及优化病毒感染性的能力已经被研究过。最近细胞转染法的改进现在允许将这些研究扩展到最相关的细胞--原代人类T淋巴细胞。T细胞活化可能影响Nef的特性。这一假说最适合在未转化的细胞中进行检验。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Study the effects of Nef on protein trafficking and viral infectivity in primary cultures of human cells. Nef is a significant contributor to the virulence of HIV-1. A variety of phenotypes associated with NEF-expression in continuous human cells lines including its ability to down-regulate CD4 and class I MHC from the cell surface and to optimize viral infectivity have been studied. Recent improvements in cell-transfection methods now allow extension of these studies to the most relevant cells, primary human T lymphocytes. T cell activation may influence the properties of Nef. This hypothesis is best tested in non-transformed cells.