NATIVE & RECOMBINANT MEMBRANE PROTEINS FOR DETERMINING FUNCTION MECHANISM

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 我们实验室的重点是在化学水平和原子结构水平上揭示完整的膜蛋白、可溶性蛋白和多肽的作用机制，以影响药物设计。膜蛋白包括GPCR、Trp、Aquaglycerol、Vglut和AMT家族的许多成员。可溶性蛋白质包括RNA加工酶、SRP蛋白、聚酮合成酶、EPO受体和设计的螺旋束。合成肽包括用于产生抗体的那些、酶底物和配体以及多肽。在表达和合成之后，这些蛋白质和多肽将在三维结晶之前被提纯和鉴定。为了便于表征和结晶，将使用质谱学来分析纯度、质量和翻译后加工事件。此外，这些努力将进一步开发适合于质谱学的环境中疏水蛋白质和多肽的纯化程序。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Our laboratory focus is to reveal functional mechanisms of integral membrane proteins, soluble proteins, and peptides at the level of chemistry and atomic structure for impact on drug design. Membrane proteins include many members of the GPCR, TRP, Aquaglycerol, Vglut, and Amt families. Soluble proteins include RNA processing enzymes, SRP proteins, Polyketide Synthases, EPO receptors, and designed helical bundles. Synthesized peptides include those used for antibody production, enzyme substrates and ligands, and peptitergents. Following expression and synthesis, these proteins and peptides will be purified and characterized prior to 3-D crystallization. To facilitate characterization and crystallization, mass spectrometry will be used to assay purity, mass, and post-translational processing events. In addition, these efforts will further develop purification procedures of hydrophobic proteins and peptides in environments amenable for mass spectrometry.