LOCALIZATION OF ADR1

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 Adr 1是一种碳源响应性锌指转录因子，是许多葡萄糖抑制基因（过氧化物酶体蛋白基因，乙醇，甘油和脂肪酸利用所需的基因）转录所必需的。当细胞在葡萄糖存在下生长时，Adr 1存在但无活性。启动子的结合需要AMP激活的蛋白激酶Snf 1，它在没有葡萄糖的情况下被磷酸化和激活。将使用在低拷贝质粒上用Adr 1-GFP转化的突变酵母细胞的荧光显微镜来确定突变是否改变Adr 1-GFP的细胞内分布。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Adr1 is a carbon source-responsive zinc-finger transcription factor, required for transcription of a number of glucose-repressed genes (peroxisomal protein genes, genes required for ethanol, glycerol, and fatty acid utilization). Adr1 is present but inactive when cells are grown in the presence of glucose. Promoter binding by requires the AMP-activated protein kinase Snf1 which is phosphorylated and activated in the absence of glucose. Fluorescence microscopy of mutant yeast cells transformed with Adr1-GFP on a low-copy plasmid will be used to determine whether the mutations alter the intracellular distribution of Adr1-GFP.