QUESTION OR TRAINING REQUEST FOR THE YEAST RESOURCE CENTER

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 嗨，我正在使用Hudson等人描述的您的Y2H系统。1997年。它工作得很好，但我在用蛋白质印迹检测蛋白质时遇到了问题。我前段时间问过你，你用的是哪种抗体来检测融合产物。我得到了回复，你说，你知道唯一有效的抗体是来自圣克鲁斯的：SC-1663。我现在购买了这个抗体，我想知道你是否可以告诉我你使用的sc-1663稀释了多少，持续了多长时间，使用了哪种二抗。非常感谢你的帮助！

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Hi, I am using your Y2H system described in Hudson et al. 1997. It works fine, but I have problems to detect the proteins by western blot. I asked you some time ago, which antibody you are using to detect the fusion products. I got a reply, where you said, that the only antibody that you know is working is from santa cruz: sc-1663. I now purchased this antibody and would like to know if you could tell me how much diluted, for how long and with which secondary antibody you are using the sc-1663. Thanks a lot for you help!