GELKEYS: A SOFTWARE APPLICATION FOR 2D GEL IMAGE STORAGE, MARKUP AND SHARING

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 我们已经创建了一个多用户基于网络的工具，用于存储2D凝胶和相关的MS数据。对于每个上传的图像，可以通过点击凝胶的表示来标记点;对于每个标记的点，可以存储多个文件。保存的图像和文件可以配置为仅限制某些组或用户的访问，允许协作，同时不公开发布数据。由于该应用程序是基于网络的，因此各团体可以在任何可以访问互联网的地方共享数据。图像可以以各种格式（PNG，GIF，TIF等）上传，也可以下载标记和不标记。GelKeys最好被视为打印和标记凝胶图像的替代方案，同时保存和组织数据以供演示。 该软件使用ActiveState Komodo 3.5在Perl中开发。使用GD和ImageMagick Perl库完成图像操作。所有图像和数据都存储在MySQL 5.0数据库中，并在Apache 2.0 Web服务器上呈现。 支持基本分析，如图像混合，该软件包括自动点定量和MS数据处理等功能。 该软件提供了一个易于使用的界面，用于存储和标记2D凝胶图像上的斑点。支持多个用户和强大的安全模型，允许个人和团体之间共享数据。手稿正在准备中。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We have created a multi-user web based tool for storing 2D gels and associated MS data. For every uploaded image, spots can be labeled by clicking on a representation of the gel; for each labeled spot, multiple files can be stored. Saved images and files can be configured to restrict access to only certain groups or users, allowing for collaboration while not publicly releasing data. As the application is web-based, groups can share data wherever they can access the Internet. Images can be uploaded in a variety of formats (PNG, GIF, TIF, etc), and can also be downloaded with markup and without. GelKeys can best be considered as an alternative to printing out and marking up images of gels, while simultaneously saving and organizing data for presentation. The software was developed in Perl using ActiveState Komodo 3.5. Image manipulation is accomplished using the GD and ImageMagick Perl libraries. All images and data are stored in a MySQL 5.0 database, and presented on an Apache 2.0 web server. Support for basic analysis such as image blending is included, and the software includes features such as automatic spot quantitation and MS data processing. The software provides an easy-to-use interface for storing and labeling spots on 2D gel images. Support for multiple users and a robust security model allow for sharing of data between individuals and groups. A manuscript is in preparation.