X-RAY FIBER DIFFRACTION OF THE OPTIC NERVE HEAD & ITS MICROFIBRILLAR COMPONENTS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 广角X射线散射衍射可以测量组织内纤维的亚微米结构和取向，这可能是功能、疾病发展和计算模型所必需的。在BioCAT对固定的猪眼组织进行蜡术，以绘制视神经头及其周围组织的胶原纤维结构(包括纤维直径、间距、相对丰度和主要方向)。数据分析正在进行中。校准后的图像被用来计算分子的D间距、丰度和定向度，如定性所示。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Wide-angle X-Ray scattering diffraction can measure the submicron structure and orientation of fibers within tissue that may be essential for function, development of disease and computational models. WAX was performed at BioCAT on fixed pig eye tissue to map the collagen fiber structure (including fiber diameter, spacing, relative abundance, and predominate orientation) of the optic nerve head and surrounding tissue. Analysis of the data is underway. The calibrated images are used to calculate the molecular D-spacing, abundance and orientation of each fibril component as qualitatively shown.