BIODEGRADATION BY WHITE ROT PHANEROCHATE CHRYSOSPORIUM

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 木质纤维素生物质是植物细胞壁中不可食用的纤维部分，作为糖的来源具有巨大的潜力，可用作平台化学品以制造燃料，材料和其他化学品。然而，生物质的复杂结构和组成阻碍了其有效转化为糖。虽然许多真菌降解部分生物质，但白色腐菌具有完全降解生物质的能力。我们计划捕捉的降解策略，然后优化的关键成分，为工业应用。 正在使用质谱法鉴定黄孢原毛平革菌在降解过程中分泌的代谢物和酶，并通过光学、扫描电子显微镜和扫描微探针拉曼显微镜（绘制木脂素/全纤维素组成的变化）和扫描微探针等技术的组合来研究其相关的降解效应。X射线衍射（SMX）（绘制纤维素纤维的分布、取向、尺寸和结晶度）。APS是北美唯一适合SMX测量的设施。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Lignocellulosic biomass, the inedible fibrous part of plant cell walls, has great potential as a source of sugars that could be used as platform chemicals to make fuels, materials and other chemicals. However, the complex structure and composition of biomass impedes its efficient conversion into sugars. Although many fungi degrade parts of biomass, white rot are unique in their ability to degrade it completely. We plan to capture the degradation strategies of Phanerochaete chrysosporium and then to optimize key components for industrial application. Metabolites and enzymes secreted by P.chrysosporium during degradation are being identified using mass spectrometry, and their associated degradation effects are being investigated by a combination of techniques including optical, SEM and scanning microprobe Raman microscopy (mapping variations in lignan/holocellulose composition) and scanning microprobe X-ray diffraction (SMX) (mapping the distribution, orientation, size and crystallinity of cellulose fibers). The APS is the only facility in North America suitable for the SMX measurements.