THE UBIQUITIN PROTEASOME SYSTEM (UPS)

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 泛素蛋白酶体系统(UPS)是由一系列依次作用的酶组成，分别命名为E1(泛素激活酶)、E2(泛素结合酶)和E3S(泛素连接酶)。UPS级联的作用负责消除泛素化的蛋白质，包括许多调节细胞周期进程的蛋白质。细胞周期底物的磷酸化允许它们被E3酶的子集识别。然后将多泛素链添加到底物上，底物将它们定位于26S蛋白酶体进行降解。在我的实验室里，我们感兴趣的是了解泛素转移的催化机制，UPS成分被调控的机制，以及E3酶识别其底物的机制。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The ubiquitin proteasome system (UPS) is made up of a sequentially acting set of enzymes termed E1(ubiquitin activating enzyme), E2 (ubiquitin conjugating enzyme), and E3s (ubiquitin ligase). The action of the UPS cascade is responsible for the elimination of ubiquitinated proteins including many that regulate cell cycle progression. The phosphorylation of cell cycle substrates allows for their recognition by a subset of E3 enzymes. Polyubiquitin chains are then added to the substrate which targets them to the 26S proteasome for degradation. In my lab we are interested in understanding the catalytic mechanism of ubiquitin transfer, the mechanism by which UPS components are regulated and the mechanism by which E3 enzymes recognize their substrates.