VIRULENCE GENE REGULATION IN STAPHYLOCOCCUS AND IDENTIFICATION OF SMALL MOLECUL

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 金黄色葡萄球菌是主要的动物病原体之一，仍然是一个主要的公共卫生问题；由于缺乏有效的疫苗，以及在社区相关或医院相关的多种抗生素耐药菌株的出现。葡萄球菌的发病是一个复杂的多因素过程，涉及多个调控系统。调控系统的功能或基本生物学过程的转录调控是基于DNA-蛋白质和蛋白质-蛋白质对上游启动子区域的相互作用；因此，了解参与基因调控的网络的作用将有助于开发新的药物来控制和预防葡萄球菌感染。其中一个调控系统是葡萄球菌中的SarA蛋白家族。在所有已测序的金黄色葡萄球菌和表皮葡萄球菌基因组中，几乎都发现了10个SARA类似基因(SarA、SarR-V、SarX和Z、MGRA和Rot)。这些蛋白质调控许多基因，涉及调控、毒力、自溶、生物膜形成和代谢过程。调控这一基因家族及其靶基因的确切机制是我们实验室的研究重点。此外，我们还致力于开发金属氧化物纳米颗粒，以开发潜在的制剂，以控制和防止细菌在社区和医院环境中传播。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Staphylococcus aureus is one of the major animal pathogens that continues to be a main public health concern; due to the lack of an effective vaccine and the emergence of multiple antibiotic-resistant strains either in community associated or in hospital associated. Staphylococcal pathogenesis is a complex multifactorial process, which involves multiple regulatory systems. The function of the regulatory systems or the transcriptional regulation of the basic biological process is based on DNA-protein and protein-protein interactions to the upstream promoter region; therefore, understanding the role of the networks involved in gene regulation will be useful for the development of new agents to control and prevent staphylococcal infections. One of these regulatory systems is the SarA protein family in Staphylococcus. A total of ten SarA paralogs (SarA, SarR-V, SarX and Z, MgrA and Rot) are found almost in all sequenced S. aureus and S. epidermidis genomes. These proteins regulate many genes involved in regulation, virulence, autolysis, biofilm formation and metabolic processes. The precise mechanisms, by which this family of genes are regulated and regulate its target genes, are the research focuses in our laboratory. Apart from these, we are also working on metal oxide nanoparticles to develop potential agents, which will control and prevent the bacterial spreading in the community and hospital settings.