Genomic and Functional Analyses of Regulatory Regions in Vertebrate Sequences

双向启动子被定义为位于两个方向相反的基因之间的共享调控区域，它们之间的距离不超过1000 bp。这些基因以头对头的排列排列，彼此分开转录。这样一对基因的转录起始位点（tss）的紧密排列被认为是基因组中的非随机事件，事实证明，比预期数量更多的启动子具有这种结构。产生双向基因对的一种可能的情况是染色体重组使两个tss的末端非常接近。这种形成很可能是不可逆的，因为连接（在双向启动子内或附近）的断裂将中断两个基因的正常调节，深刻影响正常的基因组功能。我们已经在许多脊椎动物基因组中绘制了双向启动子。在这样做的过程中，我们正在创建脊椎动物谱系中启动子序列的第一个高可信度调控图。

A bidirectional promoter is defined as the shared regulatory region that falls in the intergenic space between two oppositely oriented genes, which are separated by no more than 1,000 bp. These genes are organized in a head-to-head arrangement and transcribed away from one another. The closely spaced arrangement of the transcription start sites (TSSs) for such a pair of genes is recognized as a nonrandom event in the genome, proven by the fact that a greater than expected number of promoters have this architecture. One plausible scenario for the creation of bidirectional gene pairs is chromosomal recombination bringing the ends of two TSSs in close proximity. This formation is likely to be irreversible because breakage of the union (within or near the bidirectional promoter) would interrupt the normal regulation of two genes profoundly affecting normal genomic function. We have mapped bidirectional promoters in numerous vertebrate genomes. In doing so, we are creating the first high-confidence regulatory map of promoter sequences in vertebrate lineages. In addition to characterizing promoter regions, we are interested in identifying novel types of elements such as negative regulators of gene expression (NREs). In contrast to the large body of literature on positively acting elements such as enhancers and promoters, cis-acting NREs have not been extensively studied. Despite their scarcity in the literature, these elements are likely to be abundant in the genome. Examples of NREs include silencers, which decrease expression of a gene under their regulation and enhancer-blocking (EB) elements, which prevent the action of an enhancer on a promoter when placed between the two, but not otherwise. By developing a strategy to experimentally identify NREs, we have identified novel elements with these functions in the human genome. Exonic splicing enhancers (ESEs) are a third category of regulatory elements affecting gene expression. We have compared all predictive methods for ESEs to show that some predict sites more successfully than others. WE have produced an online toolkit to test polymorphisms that occur in coding sequences to assess whether they may affect mRNA splicing. All of these research projects examine cis-acting elements and converge on the identification of transcription factor binding sites or splicing regulators, which are bound by trans-acting proteins, together representing the basic components of gene regulation. Regulatory motifs have been published for each of the projects above. During the course of our work, novel motifs were implicated as silencers and new biological insights were revealed for the regulation of alternative promoters. A collaborative effort has been established to define the regulatory networks involving these motifs, especially in biological pathways implicated in cancer. Moreover, we are building tools to assess epigenetic events that aberrantly affect gene expression in these same pathways in tumors versus normal cells.