Live-cell Wavefront Metrology

对活的生物体的研究越来越多地建立在对活的有机体内发生的过程的动力学的研究上，而不是像过去那样，研究死亡的、染色的、脱水的和切片的生物。波前传感技术用于测量大气湍流对天文学高精度成像的影响，为跟踪活细胞成像中用作示踪剂的荧光粒子频率提供了一种机制。这种生物学应用与天文学有许多共同之处--可用的光线水平很低(恒星微弱，明亮的荧光颗粒毒害细胞)，观测可能会受到时间和空间变化的扭曲(由于天文学中的大气，由于活细胞成像中的标本本身)，根据应用的不同，可能需要使用不同的光谱(颜色)区域或光谱带。该方案寻求使用为天文学开发的波前传感技术来解决生物学中三维和时变过程的成像问题。许多活体标本的发育研究将受益于这些技术的成功应用。

The study of living organisms is increasingly based on studies of the dynamics of processes occurring within the living organism rather than, as in the past, the study of dead, stained, dehydrated and sectioned secimens. Wavefront sensing techniques used to measure the effects of atmospheric turbulence on high-precision imaging in astronomy provide a mechanism for the tracking of the fluorescent particles frequenlty used as tracers in live-cell imaging. This biology application has much in common with astronomy - the light levels available are low (stars are faint, bright fluorescent particles poisen the cells), the observation may be subject to time and space-varying distortions (due to the atmosphere in astronomy, due to the specimen itself in live-cell imaging), dependent on the application, different spectral (colour) regions or spectral bands may need to be used. This programme seeks to use wavefront sensing techniques developed for use in astronomy to the problems of imaging 3-dimensional and time-varying processes in biology. Many developmental studies of living specimens will benefit from a successful application of these techniques.