Conformal Sealant for Burn Wound Repair

描述（由申请人提供）：深度部分厚度烧伤涉及真皮较深层，通常至少2至3周不会愈合。这通常会导致明显的疤痕和挛缩，尤其是在儿童中。对于深度部分厚度烧伤尚无既定的标准治疗方法。一般来说，可以用封闭敷料或局部抗菌（例如，西尔瓦丁或杆菌肽）乳膏和吸收敷料覆盖；显然，这些都是临时使用的，没有一个能促进愈合。烧伤治疗尚未遇到的挑战是设计一种预防感染，加速愈合和减少疤痕的补救措施。在本项目中，我们将研制一种可成型/适形的抗菌组织密封敷料，用于治疗烧伤创面。同时，我们将使用水凝胶在部署时最可能密切接触的模型细胞类型来验证每种组织密封剂配方缺乏细胞毒性潜力，然后进行体内生物相容性测试。抗菌活性也将被测试，随后将在猪皮肤烧伤中进行体内功效验证研究。

DESCRIPTION (provided by applicant): Deep partial thickness burns involve the deeper layers of the dermis, and usually do not heal for at least 2 to 3 weeks. This often results in significant scarring and contractures, especially in children. There is no established standard treatment for deep partial thickness burns. In general, they may be covered with an occlusive dressing or a topical antimicrobial (e.g., Silvadene or bacitracin) cream and an absorptive dressing; evidently, these are all applied on an ad hoc basis and none of them promotes healing. The unmet challenge of burn treatment is to devise a remedy that prevents infection, speeds healing and reduces scarring. In this project, we will formulate a formable/conformable antibacterial tissue sealant dressing specific for treating burn wounds. In parallel, we will validate the lack of cytotoxicity potential of each tissue sealant formulation using model cell types that the hydrogel will most likely be in close contact upon deployment, to be followed by in vivo biocompatibility testing. The anti-bacterial activities will also be tested, to be followed by an in vivo efficacy validation study in porcine dermal burns. PUBLIC HEALTH RELEVANCE: Burns are one of the most common causes of injury in all age groups. The primary goals of acute burn wound management are prevention of infection and the promotion of optimal wound closure by re-epithelialization to minimize aesthetic and functional impairment. Deep partial thickness burns involve the deeper layers of the dermis, and usually do not heal for at least 2 to 3 weeks. This often results in significant scarring and contractures, especially in children. There is no established standard treatment for deep partial thickness burns. In general, these burns may be covered with an occlusive dressing or a topical antimicrobial (e.g., Silvadene or bacitracin) cream and an absorptive dressing; evidently, these are all applied on an ad hoc basis and none of them promotes healing. The unmet challenge of burn treatment is to devise a remedy that prevents infection, speeds healing and reduces scarring. It is our intention to develop an antibacterial tissue sealant capable of sealing the burn wound, preventing infection, accelerating wound healing and reducing scarring.