Manufacture of Plastic Fiber Optic Microarray Plates for Gene Sequencing

描述（由申请人提供）：这个小企业创新研究第一阶段项目将研究和开发一个基于塑料纤维微阵列板的平版印刷生产的制造平台。与现有的玻璃纤维微阵列板相比，这些板将具有重要的优势：基因测序、生物芯片、微量滴定板、微和微微孔板、微流体阵列和微毛细管阵列的首选平台。玻璃纤维板已被广泛用于执行指示特定生化反应的荧光信号的光学读出。在第1阶段，将研究和优化拟定的单体材料。所选择的材料将聚合成光纤微阵列板的结构形式。将研究该板的机械和光学性质，以证明这种板的必要特性。该平板将在现有的商业基因测序系统中操作。在第二阶段，该板技术将商业化。这项工作的广泛的，长期的目标是开发必要的自动化制造技术的光学读出的下一代基因测序系统的反应体积预计具有亚微米尺寸。塑料纤维微阵列板将实现相对于反应体积的亚微米对准，最高可能的光学灵敏度，并满足“1000美元全人类基因组”挑战所施加的价格点。使用新的光刻技术和新材料是唯一已知的方法，以满足这些广泛的目标。快速而廉价地对DNA进行测序的手段将为科学、医学和制药业带来重大进步。特别是，以负担得起的价格对整个人类基因组进行测序的能力将使医疗保健能够根据个人的特定需求进行量身定制，引发个性化医疗的革命。 公共卫生相关性：以可承受的价格对整个人类基因组进行测序的能力将使医疗保健能够根据个人的特定需求进行定制，引发个性化医疗的革命。

DESCRIPTION (provided by applicant): This Small Business Innovative Research Phase 1 project will research and develop an enabling manufacturing platform based on lithographic production of plastic fiber microarray plates. These plates will have important advantages over existing glass-fiber microarray plates: the platform of choice for gene sequencing, biochips, microtiter plates, micro and picowell plates, microfluidic arrays and microcapillary arrays. The glass fiber plates have been extensively used to perform optical readout of fluorescence signals indicative of specific biochemical reactions. In Phase 1, the proposed monomer materials will be studied and optimized. The selected materials will be polymerized into the structural form of a fiber optic microarray plate. The mechanical and optical properties of the plate will be studied to demonstrate the necessary characteristics of such a plate. The plate will be operated in an existing commercial gene sequencing system. In Phase 2, that plate technology will be commercialized. The broad, long term objectives of this work are to develop the necessary automated manufacturing technology for optical readout of next generation gene sequencing systems where reaction volumes are expected to have submicron dimensions. The plastic fiber microarray plates will achieve submicron alignment relative to reaction volumes, the highest possible optical sensitivity, and meet the price point imposed by the "$1000 whole human genome" challenge. The use of novel lithographic techniques and new materials are the only known approach to meet these broad objectives. The means to rapidly and inexpensively sequence DNA will bring about major advances in science, medicine and the pharmaceutical industry. In particular, the ability to sequence the entire human genome at an affordable price will allow healthcare to be tailored to the specific needs of an individual, trigging a revolution in personalized medicine. PUBLIC HEALTH RELEVANCE: The ability to sequence the entire human genome at an affordable price will allow healthcare to be tailored to the specific needs of an individual, trigging a revolution in personalized medicine.