FACSAria II Cell Sorter for Non-Biohazardous Cells

描述（由申请人提供）：细胞分选技术对生物医学领域的研究越来越重要，包括基础发育生物学、癌症生物学、微生物学和再生医学。宾夕法尼亚大学的研究人员，包括宾夕法尼亚大学的Abramson癌症中心，宾夕法尼亚大学医院，宾夕法尼亚大学医学院和费城儿童医院，正在进行需要多参数流分选能力的研究项目。这项技术对于这些机构正在进行的许多NIH资助的研究项目的生产力变得尤为重要。目前对分类的需求超过了现有仪器的能力。该项目要求购买一台仪器，以推进现有技术，降低运营成本，并取代过时的分拣机。此外，Becton Dickinson FACSAria II专门用于分选非人类细胞，将促进宾夕法尼亚大学校园内许多科学家的研究计划。该提案中的16个主要用户拥有各种NIH资助的研究项目，这些项目将从购买该仪器中受益。强有力的机构支助、技术专门知识和管理计划已经到位，以确保有效利用这一资源。 公共卫生相关性：在分离可用于生物医学研究的活单细胞的仪器能力方面有了重大改进。该项目支持购买一种仪器，专门用于从宾夕法尼亚大学用于开发新疗法的各种动物细胞中分离细胞。

DESCRIPTION (provided by applicant): Cell sorting technology is increasingly important to research in the biomedical field, including basic developmental biology, cancer biology, microbiology and regenerative medicine. Researchers at the University of Pennsylvania, including the Abramson Cancer Center of the University of Pennsylvania, the Hospital of the University of Pennsylvania, the University of Pennsylvania School of Medicine, and the Children's Hospital of Philadelphia, are pursuing research projects that require multiparameter flow sorting capabilities. This technology is becoming particularly critical to the productivity of many ongoing NIH-funded research projects at these institutions. The demand for sorting currently exceeds the capacity of available instruments. This project requests the purchase of an instrument that will advance existing technology, decrease operating costs and replace an outdated sorter. The addition of a Becton Dickinson FACSAria II that is dedicated to sorting non-human cells will facilitate the research programs of many scientists on campus at the University of Pennsylvania. The 16 major users in this proposal have a wide variety of NIH-funded research projects that will benefit from the purchase of this instrument. Strong institutional support, technical expertise and a management plan are in place to assure the efficient utilization of this resource. PUBLIC HEALTH RELEVANCE: There have been major improvements in the ability of instruments to isolate live single cells that can be used for biomedical research. This project supports the purchase of an instrument that is dedicated to isolate cells from a variety of animal cells that are being used to develop new therapies at the University of Pennsylvania.