600MHz Varian VNMRS Console Upgrade

描述（由申请人提供）：本申请的核磁共振（NMR）光谱仪主题支持UT西南医学中心众多研究人员的广泛研究。它允许研究医学和生物学相关系统的蛋白质结构，动力学和分子相互作用。例如，Michael罗森博士研究细胞骨架动力学，这是一个重要的生物学过程，涉及细胞迁移、粘附、极性的建立和维持以及囊泡运输。Jose Rizo-Rey博士旨在了解参与膜融合的蛋白质对神经递质释放的调节。Kevin Gardner博士对环境信号的信号转导感兴趣，如外源性物质，光和氧气最终参与基因调控。Xuelian Luo博士正在描述细胞有丝分裂期间纺锤体检查点信号传导的调节，细胞周期的染色体遗传点。这些系统对基础生物学和开发诊断和治疗人类疾病的新方法都很重要。核磁共振光谱仪已经使用了10年以上，使用的技术不再由仪器制造商生产。维修变得越来越频繁和昂贵，需要更长的时间，很快就不可能了。该提案要求提供资金，以购买一个新的600兆赫核磁共振波谱仪控制台。控制台是核磁共振光谱仪的核心，因为它包含了获取核磁共振实验所需的所有数字和射频电子设备。该设备将继续支持Michael罗森博士、Jose Rizo-Rey博士、Kevin Gardner博士和Xuelian Luo博士实验室的NIH资助项目，从而促进上述研究。此外，该设备也将提供给UT西南社区需要核磁共振数据的生物物理研究更广泛的重要生物过程。

DESCRIPTION (provided by applicant): The nuclear magnetic resonance (NMR) spectrometer subject of this application supports a wide range of research among numerous investigators at UT Southwestern Medical Center. It allows the study of protein structure, dynamics, and molecular interactions on medically and biologically relevant systems. For example, Dr. Michael Rosen studies cytoskeletal dynamics, an important biological process involved in cell migration, adhesion, establishment and maintenance of polarity, and vesicle trafficking. Dr. Jose Rizo-Rey aims to understand the regulation of neurotransmitter release by proteins involved in membrane fusion. Dr. Kevin Gardner is interested in how signal transduction of environmental cues, like xenobiotics, light, and oxygen are ultimately involved in gene regulation. Dr. Xuelian Luo is characterizing the regulation of the spindle checkpoint signaling during cell mitosis, the chromosome inheritance point of the cell cycle. These systems are important to basic biology and to developing new methods to diagnose and treat human disease. The NMR spectrometer is over 10 years old, and uses technology no longer produced by the instrument manufacturer. Repairs are increasingly frequent and expensive, take longer, and soon will not be possible. This proposal requests funds to purchase a new 600MHz NMR spectrometer console. The console is the heart of a NMR spectrometer since it contains all the digital and radiofrequency electronics necessary to acquire NMR experiments. This equipment will continue supporting NIH-funded programs in the laboratories of Dr. Michael Rosen, Dr. Jose Rizo-Rey, Dr. Kevin Gardner, and Dr. Xuelian Luo, thus facilitating the above stated research. Additionally, this equipment will also be available to the UT Southwestern community in need of NMR data for biophysical studies on an even wider range of important biological processes.