Nikon A1 laser scanning confocal microscope equipped for live imaging of cells an

描述（由申请人提供）：共聚焦显微镜的实时成像揭示了生物过程的新的和令人兴奋的特征，并加强了许多美国国立卫生研究院资助的研究项目。由于每次实验都需要花费大量的时间，像马萨诸塞州总医院的查尔斯敦海军基地这样的大型研究机构对共聚焦显微镜的需求越来越大。本方案涵盖校区149号楼三个系的7个主要用户。这些研究人员将使用该机器进行细胞和组织水平的实时成像，以及标准的4通道共聚焦显微镜。首选的尼康A1共焦将配备共振和非共振扫描仪，用于高速和高分辨率扫描。在延时成像期间，机动阶段将有助于在多个区域收集数据，而加热阶段将允许对哺乳动物细胞进行长期成像。15个主要和次要用户实验室的研究项目相当多样化，但他们都需要在共聚焦显微镜上配置实时成像和最先进的4通道共聚焦显微镜。

DESCRIPTION (provided by applicant): Live imaging by confocal microscopy has revealed new and exciting features of biological processes, and enhanced many NIH-funded research programs. Because of the time involved in each experiment, live imaging projects generate an increased need for confocal microscopes in large research facilities like the Charlestown Navy Yard campus of the Massachusetts General Hospital. This proposal covers 7 major users from three departments located in Building 149 on this campus. These researchers will use the machine for live imaging at the cell and tissue level, as well as for standard 4 channel confocal microscopy. The preferred Nikon A1 confocal will be equipped with both resonant and non-resonant scanners for high speed and high resolution scanning. A motorized stage will facilitate data collection at multiple regions during time lapse imaging, and a heated stage will allow for long term imaging of mammalian cells. The research programs of the fifteen major and minor user labs are quite diverse, but they share a need for imaging on a confocal microscope configured for live imaging and state-of-the-art 4 channel confocal microscopy.