JEOL JEM-1400 Transmission Electron Microscope

描述(由申请人提供)：沃兹沃斯中心的一组研究人员正在申请资金，以购买JEOL JEM-1400透射电子显微镜(TEM)。该仪器的主要用户包括美国国立卫生研究院资助的五个实验室，这些实验室有广泛的记录，其具体目标需要使用多种常规薄片和低温方法进行电子显微镜分析。直到2008年底，沃兹沃斯的两台显微镜都可以用于这些项目。其中一架已有24年历史的飞利浦EM420已到了使用寿命的尽头，并于2008年底退役。另一种是已有14年历史的蔡司910，尽管有全面的服务计划，但它已经变得如此不可靠，以至于它的停机时间(2008年为66%)成为研究进展的重大障碍。此外，蔡司910不能用于以前在EM420上执行的低温工作。JEOL JEM-1400是蔡司910的理想替代品，因为它可以很容易地适应传统和低温操作模式。该仪器是至关重要的，因为它可以接受一次可以加载多达五个栅格的标本夹持器。对于执行广泛序列部分分析的三个主要用户来说，此功能代表着显著的功能改进。还包括一台性价比高的2k x 2k数码相机，特别是用于断层扫描和单粒子低温电磁项目。这一工具将被整合到一个长期存在的EM核心中，由地方行政和监督委员会确保适当的使用和维护。沃兹沃斯中心坚定地致力于增加JEM-1400。作为支持的证据，该中心将提供一名国家资助的、经验丰富的研究科学家来监督仪器的运行，承担与年度服务合同相关的费用，并提供额外资金来购买五个主要用户中的三个所需的低温工作台、控制器和干式泵站设备。此外，在时间允许的情况下，该仪器将在专门的EM核心的协助下提供给其他资助的研究人员，从而加强沃兹沃斯中心长期以来将电子显微镜作为研究工具的使用。

DESCRIPTION (provided by applicant): A group of investigators at the Wadsworth Center are requesting funds to purchase a JEOL JEM-1400 transmission electron microscope (TEM). The major users of this instrument include five NIH-funded laboratories with extensive track records in, and whose specific aims require analyses by electron microscopy using multiple conventional thin-section and cryo approaches. Until the end of 2008, two microscopes at Wadsworth were available for these projects. One of these, a 24-year-old Philips EM420, has reached the end of its service life, and was decommissioned at the end of 2008. The other TEM, a 14- year-old Zeiss 910 that, despite being covered by a comprehensive service plan, has become so unreliable that its downtime (66% in 2008) represents a significant barrier to research progress. Moreover, the Zeiss 910 cannot be used for the cryo work formerly performed on the EM420. The JEOL JEM-1400 is an ideal replacement for the Zeiss 910, because it will readily accommodate both conventional and cryo modes of operation. This instrument is vital as it accepts a specimen holder that can load up to five grids at one time. For the three major users that perform extensive serial-section analyses, this feature represents a significant functional improvement. A cost-effective 2k x 2k digital camera is also included, particularly for the tomographic and single-particle cryo-EM projects. This instrument will be integrated into a long- standing EM Core, with local administrative and oversight committees to ensure appropriate use and maintenance. The Wadsworth Center is strongly committed to the addition of the JEM-1400. As evidence of support, the Center will provide a State-funded, experienced, Research Scientist to oversee the instrument's operation, assume costs associated with the annual service contract, and provide additional funds to purchase the cryo stage, controller, and dry pumping station equipment that are required for three of the five major users. In addition, and as time permits, this instrument will be made available to other funded investigators with assistance from a dedicated EM Core, thus enhancing the Wadsworth Center's long-standing use of electron microscopy as a research tool.