Shared Equipment Grant for Purchase of a Leica TCS SP5 Confocal Microscope

描述(由申请者提供)：本建议书申请购买徕卡TCS SP5共聚焦显微镜的资金。徕卡TCS SP5是一款单点宽带共焦模块化平台，配备了RGB(HENE 633 nm、HENE 594 nm、HENE 543、Argon 458/476/488/496/514)和UV二极管激光器(405 Nm)。请拨这笔钱是为了升级SBU发育遗传学中心的共焦设施。CDG研究人员目前可用的SP2系统已有八年多的历史，不再满足该中心的技术要求。SP5将解决目前影响我们研究人员的几个困难，从而促进来自石溪大学(SBU)的九位杰出科学家的研究目标和生产率。该系统将为研究人员提供更高的灵敏度、快速扫描能力和一条用于成像、光激活和FRET实验的UV激光线路，从而使该中心保持竞争力。共焦系统的管理和操作将由一名技术专家提供，他还将协助用户制定特殊项目的染色和成像方案，并协助调查人员分析共焦数据。

DESCRIPTION (provided by applicant): This proposal requests funds for the purchase of a Leica TCS SP5 confocal microscope. The Leica TCS SP5 is a single point broadband confocal modular platform equipped with an RGB (HeNe 633nm, HeNe 594nm, HeNe 543, Argon 458/476/488/496/514) and a UV-diode laser (405 nm). It is being requested for the purpose of upgrading the confocal facility at the SBU Center for Developmental Genetics (CDG). The current SP2 system available to the CDG researchers is over eight years old and no longer meets the technical requirements of the center. The SP5 will address several difficulties currently affecting our researchers, thereby promoting the research objectives and productivity of nine distinguished scientists from Stony Brook University (SBU). The system will allow the center to remain competitive by providing researchers with increased sensitivity, fast-scanning capabilities, and a UV laser line for imaging, photo-activation, and FRET experiments. Management and operation of the confocal system will be provided by a technical specialist, who will also assist users in developing staining and imaging protocols for special projects, as well helping investigators in the analysis of confocal data.