Flow Cytometer / Cell Sorter

描述(申请人提供)：本申请申请资金用于购买BD FACSAria II流式细胞仪/细胞分选仪。这一应用源于当前流式细胞仪的崩溃，以及福赛斯8个部门中5个部门的13名调查人员对流式细胞仪细胞分类服务日益增长的需求。在过去的二十年里，流式细胞仪已经成为Forsyth研究项目的宝贵工具，来自不同实验室的大量出版物表明，该仪器是实现研究目标不可或缺的组成部分。这些研究人员对多路分析功能(60%)和细胞分选(40%)的需求不能满足目前已过时的EPICS ALTRA流式细胞仪(已有10年历史)。附近(至少2英里外)的其他设施无法满足我们研究人员的日常使用需求，原因是：1)其他研究所的容量饱和导致使用时间有限；2)其他研究所产生的昂贵服务费，特别是在分类方面；3)往返运送样本，导致担心污染和细胞存活。调查人员经常对未能及时可靠地处理珍贵的细胞样本表示失望。这项申请的参与者代表了该研究所由NIH资助的研究人员的大多数(56%)，而将从新工具中受益的项目代表了NIH授予Forsyth的大部分拨款(59%)。因此，所要求的文书将确保最佳利用现有国家卫生研究院支持的调查人员提供的资金。仪器的使用将由首席调查员、流式细胞术/细胞分选核心主任和其他1名在流式细胞术方面具有丰富经验的后备协调员管理，并由一个咨询委员会通过一个由5名经验丰富的调查员组成的小组进行监测。这些研究人员还将与其他现有或新用户分享他们在流式细胞仪方面的专业知识，以充分利用该仪器来加强他们的研究项目。将招聘一名指定的技术员，在PI和核心主任的监督下负责仪器的日常操作和维护。Forsyth已表示坚决承诺在人员支持、空间、计算机和网络支持以及提供服务合同方面支持该工具。 与公共卫生相关：所要求的流式细胞仪/细胞分选仪能够每秒分析流体中的数千个微观颗粒，并能主动分离和分离具有特定性质的细胞。该技术已成为许多生物医学研究的重要工具，并在研究中有广泛的应用，包括分子生物学、病理学、免疫学、微生物学，以及医学，如移植、血液学、肿瘤免疫和化疗以及人类遗传学。

DESCRIPTION (provided by applicant): This application requests funds for the purchase of a BD FACSAria II flow cytometer / cell sorter. This application arises from the breakdown of the current flow cytometer, and from a growing demand for flow cytometric cell sorting services among a user group of thirteen investigators from five of the eight departments at Forsyth. In the past two decades, flow cytometry has become an invaluable tool for the research projects at Forsyth and numerous publications from different laboratories demonstrate this instrument as an indispensible component to achieve the research goals. These investigators' needs for multiplexing analysis functions (60%) and cell sorting (40%) are not satisfied by the obsolete EPICS Altra flow cytometer (10 years old) currently in place. Other facilities in the vicinity (at least 2 miles away) can't meet our investigators' demand for day-to-day usage because of: 1) limited user time due to saturated capacity in other institutes; 2) pricy service fee incurred in other institutes, especially for sorting; 3) back- and-forth transportation of samples leading to concerns of contamination and cell viability. Investigators frequently express frustration for not being able to reliably process precious cell samples in a timely fashion. The participants in this application represent a majority (56%) of the NIH-funded investigators at this institute, and the projects that will benefit from the new instrument represent a majority of the NIH grant (59%) awarded to Forsyth. The requested instrument will thus assure optimal use of the funding from existing NIH-supported investigators. Use of the instrument will be managed by the principle investigator, Director of the Flow Cytometry/ Cell Sorting Core, and 1 other back-up coordinators with extensive experience in flow cytometry, and monitored by an advisory committee through a group of five experienced investigators. These investigators will also share their expertise in flow cytometry with other existing or new users to enhance their research projects by taking the full advantage of the instrument. A designated technician will be recruited and will be responsible for the routine operation and maintenance of the instrument under the supervision of the PI and Director of the core. Forsyth has expressed a firm commitment to the support of this instrument in terms of personnel support, space, computer and networking support, and provision of service contracts. PUBLIC HEALTH RELEVANCE: The requested flow cytometer / cell sorter is able to analyze thousands of microscopic particles every second, in "real time" in a stream of fluid, and can actively separate and isolate cells having specified properties. The technology has become an essential tool for many biomedical studies and has broad applications in research, including molecular biology, pathology, immunology, microbiology, as well as in medicine, such as transplantation, hematology, tumor immunology and chemotherapy, and human genetics.