IVIS Spectrum imager of bioluminescence and fluorescence

描述(由申请人提供)：将购买一个IVIS光谱成像系统，用于阿尔伯特·爱因斯坦医学院的共享小动物成像设施。IVIS成像系统允许对表达荧光素酶的小动物进行实时成像，或对注射了表达荧光素酶的细胞或微生物的动物进行成像。荧光素酶d-荧光素底物很容易渗透到细胞和组织中，导致个性化器官的生物发光成像(BLI)，并与解剖位置相关。Spectrum还可以实时成像荧光(GFP、mCherry等)。IVIS频谱将用于监测肿瘤或细胞迁移，并监测各种病原体的传播。由于可以在不牺牲动物的情况下监测荧光素酶或荧光活性，因此可以对单个小鼠进行随时间推移的纵向测量。此外，还可以获得三维图像，提供有关记者解剖定位的信息。该信号与细胞数目成正比，并且可以使用IVIS机器提供的软件容易地进行量化。这项成像技术将帮助对癌症生物学、免疫学和传染病感兴趣的研究人员。最后，购买这种仪器将使研究人员能够最大限度地减少研究所需的动物数量，因为研究中的每一种动物都将获得多幅图像。 公共卫生相关性：阿尔伯特·爱因斯坦医学院提议为共享的小动物成像设施购买一台IVIS光谱成像仪器。这一仪器将使研究人员能够监测小动物体内表达生物发光分子或荧光分子的细胞。科学家们将利用这项技术来了解癌细胞如何转移，感染性病原体如何致病，干细胞如何重新填充器官，以及哺乳动物如何对病原体或癌症做出免疫反应。

DESCRIPTION (provided by applicant): An IVIS Spectrum imaging system will be purchased for use in a shared small animal imaging facility at the Albert Einstein College of Medicine. The IVIS imaging systems allow the real time imaging of small animals expressing luciferase or imaging of animals injected with cells or microbes expressing luciferase. The luciferase d-luciferin substrate is readily permeable into cells and tissue, resulting in bioluminescence imaging (BLI) of individualized organs and correlation to anatomic location. The Spectrum can image fluorescence as well (GFP, mCherry etc) in real time. The IVIS Spectrum will be used to monitor tumor or cellular migration and to monitor dissemination of a variety of pathogens. Because the luciferase or fluorescence activity can be monitored without sacrificing animals, longitudinal measurements can be made on individual mice over time. In addition 3 dimensional images can be acquired providing information about anatomic localization of reporters. The signal is proportional to cell number and can be readily quantified using the software provided with the IVIS machinery. The imaging technique will assist investigators interested in cancer biology, immunology and infectious diseases. Finally, the purchase of this instrument will enable investigators to minimize the number of animals required for their studies, as multiple images will be acquired for each animal in a study. Public Health Relevance: The Albert Einstein College of Medicine proposes to buy an IVIS Spectrum imaging instrument for a shared small animal imaging facility. This instrument will enable investigators to monitor cells expressing bioluminescent molecules or fluorescent molecules within small animals. Scientists will use this technique to understand how cancer cells metastasize, how infectious agents cause disease, how stem cells repopulate organs, and how mammals mount an immune response to pathogens or cancer.