In vivo non-invasive 3D quantitative IVIS Spectrum molecular imaging system

描述(申请人提供)：本提案的目标是获得一种非侵入性的3D定量分子成像系统，该系统可以进行体内、体外和体外成像。该系统名为卡利珀生命科学公司的IVIS光谱，能够传输和反射荧光和生物发光成像。它有10个窄带激励滤光片和18个窄带发射滤光片，允许扫描大波长区域和评估多个记者。该设备允许对麻醉动物体内深层组织来源内的记者进行可视化。具体地说，可以用特定的发光报告标记细胞，并将其注射到小鼠体内，并最终在小鼠体内鉴定它们的定位和生长。具体的例子包括转移研究或血管生成的量化。该应用程序支持12名研究人员和13个R01的研究组合、1个P01中的4个项目、2个P50孢子中的3个项目、2个R21和1个K22奖、NIH主任奖和U奖中的项目，总共25个NIH资助的项目。该小组由多个部门以及医学和牙科学校的成员组成。目前，校园内有一个IVIS Spectrum系统，但距离癌症中心动物设施步行15分钟(运输动物尤其困难，尤其是在冬天)，而且通常需要很长的等待时间(几周)才能获得访问权限，这给计划体内实验带来了巨大的挑战，时间可能非常困难。购买这一系统的主要理由是获得这项技术，以便更好地计划体内实验，因为机器时间不那么拥挤，并使设备位于癌症中心大楼动物设施容易接近的位置，供大量研究人员使用。已经规划的项目将利用87%的年度用户时间，这表明对这种新设备的需求很高。研究项目与NIH改善健康的目标直接相关，包括癌症转移、癌症治疗、血管生成、骨生物学和与年龄相关的疾病的研究。该设备将由经验丰富的技术人员和内部咨询委员会提供支持。一个吸引和培训新用户的系统已经到位，这样NIH资助的其他调查人员将从这些设备中受益。头四年承诺为维护和技术费用提供大量机构支助，以确保该系统的长期可行性(四年期间共计151600美元)。在这段时间之后，将实施以用户为基础的充值系统，以继续长期使用。此外，还有为设备提供空间的机构承诺。总而言之，使用这一尖端成像系统将大大加快密歇根大学大量由NIH资助的研究人员的研究步伐。 公共卫生相关性：我们要求一种成像系统，使我们能够检测和量化长期生长在活动物体内的特定细胞。这将使我们能够确定疾病的机制或监测试验疗法的疗效。最终，它将使我们能够设计或优化疾病的治疗方法。

DESCRIPTION (provided by applicant): The objective of this proposal is to acquire a non-invasive 3D quantitative molecular imaging system that allows for in vivo, ex vivo and in vitro imaging. The system, called the IVIS Spectrum from Caliper LifeSciences, is capable of transmission and reflectance fluorescence and bioluminescence imaging. It has 10 narrow band excitation filters and 18 narrow band emission filters which allow for scanning over a large wavelength region and evaluation of multiple reporters. The equipment allows for visualization of reporters located within deep tissue sources in vivo in anesthetized animals. In concrete terms, cells can be labeled with specific luminescent reports and injected into mice and their eventual localization and growth identified in mice. Specific examples include metastasis studies or quantification of angiogenesis. This application supports 12 investigators and a research portfolio of 13 R01s, 4 projects in one P01, 3 projects in two P50 SPORES, 2 R21s and 1 each of a K22 award, NIH Director's Award, and project in a U award for a total of 25 NIH-funded projects. The group consists of members of multiple Departments and the Medical and Dental Schools. Currently, there is one IVIS Spectrum system on campus, but at a 15 minute walk from the Cancer Center animal facility (a particular challenge for transporting animals, especially in winter) and often has lengthy wait periods (several weeks) to obtain access resulting in great challenges to planning in vivo experiments that can be very hard to time. The principal rationale for purchase of this system is to gain access to this technology so that in vivo experiments can be better planned due to less congested access to machine time and to have the equipment in a location readily accessible to the Cancer Center Building animal facility for a large number of investigators. The projects already planned for will utilize 87% of the annual user time indicating the high demand for this new equipment. Research projects are directly related to NIH goals of improving health and include studies on cancer metastasis, cancer therapeutics, angiogenesis, bone biology and age-related disease. The equipment will be supported by an experienced technical staff and an internal advisory committee. A system to attract and train new users is in place so that other NIH-funded investigators will benefit from the equipment. Significant institutional support for both maintenance and technical costs has been committed for the initial four years to ensure long-term viability of the system (Total of $151,600 over 4 years). A user-based recharge system will be put into place after this time period to continue its long-term use. Additionally, there is institutional commitment of space for the equipment. In summary access to this cutting edge imaging system will greatly accelerate the pace of research at University of Michigan for a large number of NIH-funded investigators. Public Health Relevance: We are requesting an imaging system that allows us to detect and quantify specific cells growing within living animals over long time periods. This will allow us to identify mechanisms of disease or monitor efficacy of test therapies. Ultimately, it will enable us to design or optimize treatments for disease.