Transmission Electron Microscope

描述（由申请人提供）：本申请要求资金购买Hitachi Model H-7650 Biological，120 μ m，透射电子显微镜（TEM），配有Gatan 1100万像素CCD摄像机，用于固定生物样品的高分辨率成像。该仪器将是一个共享资源，位于斯坦福大学一个完善的多用户光学和电子显微镜设施：细胞科学成像设施（http：taltos.stanford.edu）。这个设施是访问斯坦福大学的整个研究社区以及周围的生物技术公司。所要求的TEM将支持NIH资助的九个主要用户的项目。用户组的所有成员都有将电子显微镜应用于NIH资助的研究项目的丰富经验。这些项目研究了一系列NIH支持的课题，包括：1）有髓轴突的发育（塔尔博特）; 2）线虫触觉受体神经元的机械-电转导和微管细胞骨架组织（古德曼）; 3）酵母细胞极性的发育（普林格尔）; 4）神经元迁移的分子基础（McConnell）; 5）微管细胞骨架组织和调节（Stearns）; 6）细菌染色体和复制体的3D组织（Shapiro）; 7）病毒复制、组装和从感染细胞中排出的分子机制（阿尔文）; 8）伤口愈合和皮肤再生的细胞和分子机制（Lorenz）; 9）阿尔茨海默病中淀粉样蛋白形成途径的阐明（Graef）。这些研究调查了各种模式生物和人体组织中的关键结构问题，并涵盖了对人类健康和疾病的各个方面产生影响的研究领域，从癌症和病毒发病机制到了解免疫反应和感觉生物学的分子基础。对于细胞和亚细胞结构以及分子复合物的超微结构研究，所有这些都需要具有高分辨率数字成像的基本的、可靠的120 nm透射电子显微镜，其最有效地由配备有Gatan Inc. Orius SC 1000 B相机。

DESCRIPTION (provided by applicant): This application requests funds to purchase a Hitachi Model H-7650 Biological, 120kv, transmission electron microscope (TEM) with a Gatan 11megapixel CCD camera for high resolution imaging of fixed biological samples. This instrument will be a shared resource, located in a well-established, multi-user, light and electron microscopy facility at Stanford University: the Cell Sciences Imaging Facility (http://taltos.stanford.edu). This facility is accessible to Stanford University's entire research community as well as surrounding biotech companies. The requested TEM will support NIH funded projects from nine major users. All members of the user group have extensive experience applying electron microscopy to their NIH funded research projects. These projects investigate a wide range of NIH supported topics, including, 1) development of myelinated axons (Talbot); 2) mechano-electrical transduction and microtubule cytoskeleton organization in nematode touch receptor neurons (Goodman); 3) development of cell polarity in yeast (Pringle); 4) the molecular basis of neuronal migration (McConnell); 5) microtubule cytoskeleton organization and regulation (Stearns); 6) 3D organization of the bacterial chromosome and replisome (Shapiro); 7) molecular mechanisms of viral replication, assembly and egress from infected cells (Arvin); 8) cellular and molecular mechanisms of wound healing and skin regeneration (Lorenz); 9) elucidation of amyloid formation pathway in Alzheimer's disease (Graef). These studies investigate critical structural questions in a variety of model organisms and human tissues and cover areas of research with implications for diverse aspects of human health and disease, ranging from cancer and viral pathogenesis to understanding the molecular basis of immuno-responses and sensory biology. All require, for ultrastructural study of cellular and subcellular structures and molecular complexes, a basic, reliable 120kv transmission electron microscope with high resolution digital imaging that is most effectively provided by a Hitachi Model H-7650 TEM equipped with a Gatan Inc. Orius SC1000B camera.