UAB Shared Biacore T100 Biosensor

描述(申请人提供)：定义生物分子之间分子相互作用的动力学已成为基础微生物学和免疫学研究以及转译药物发现的重要应用。这笔共享仪器拨款要求提供资金，以购买最先进的Biacore T100表面等离子体共振(SPR)系统，以建立阿拉巴马大学伯明翰分校(UAB)的共享仪器设施。该仪器将满足校园内8个主要用户(70%)的未得到满足的需求，并将集中在谢尔比跨学科生物医学大楼内，靠近主要调查人员的实验室。这一装置将取代最近搬迁的Biacore 3000仪器，并补充现有的Biacore 2000系统，该系统仅限于联合大学校区不同区域的一群专门的用户。该仪器的剩余可用部分将留给一大批小用户和外部用户，其中包括位置较近的南方研究所。因此，所要求的T100装置和设施将为更大的科学界提供一些便利。我们计划通过派遣所有主要用户实验室的指定人员到Biacore进行具体培训来加强现有的技术专长。将聘请一名具有SPR经验的专门设施经理来维护、运行、监督和进一步教育用户。一个基础广泛的内部咨询委员会将解决任何冲突，并确定对该股的长期战略支持。强有力的机构支持将确保该仪器和设施的顺利启动和长期成功。大学和学校领导层已承诺提供大量资金，提供设施工资支持和长期五年延长保修，以确保其最佳运行。使用该工具产生的使用费将用于支付管理人员的工资支助和设施费用。这一新的核心将是直接微生物学和免疫学领域的现有研究人员以及有兴趣探索详细描述分子相互作用的创新方法的其他学科的科学家的一个极好的多学科资源。收购这一仪器和建立这一新的核心将提供一个易于访问的最先进的设施，将启动一个出色的平台，以刺激新的假说，促进生物医学的发展和国家卫生研究所资助的研究在UAB的进展。

DESCRIPTION (provided by applicant): Defining the kinetics of molecular interactions between biomolecules has become an essential application for fundamental microbiology and immunology research and translational drug discovery. This shared instrumentation grant requests funding to purchase a state-of-the-art Biacore T100 surface plasmon resonance (SPR) system to establish a shared instrumentation facility at the University of Alabama at Birmingham (UAB). The instrument would serve an unmet need on campus for a base of eight major users (70%) and would be centrally located within the Shelby Interdisciplinary Biomedical Building in close proximity to the primary investigators' laboratories. This unit would replace a recently relocated Biacore 3000 instrument and complement an existing Biacore 2000 system that is restricted to a dedicated group of users in a different area of the UAB campus. The remaining availability of the instrument would be reserved for a significant pool of minor and outside users that includes the closely situated Southern Research Institute. The requested T100 unit and facility would thus provide some accessibility to the greater scientific community. We plan to enhance existing technical expertise by sending designates from all major users laboratories to Biacore for specific training. A dedicated facility manager with SPR experience will be hired to maintain, run, supervise, and further educate users. A broadly-based internal advisory committee will resolve any conflicts and identify strategic long-term support for the unit. Strong institutional support will assure the smooth start up and long-term success of the instrument and facility. Significant funds have been committed by the University and school leadership that will provide facility salary support and a long term five year extended warranty to ensure its optimal operation. User fees generated from use of the instrument will be applied towards salary support of the manager and cost of the facility. This new core would be an excellent multidisciplinary resource for established investigators in the immediate microbiology and immunology community as well as scientists in other disciplines with an interest in exploring innovative approaches for detailed characterization of molecular interactions. Acquisition of this instrument and establishment of this new core would provide a readily accessible state-of-the-art facility that would launch an outstanding platform for stimulating novel hypotheses and enhancing biomedical growth and advancement of NIH-funded research at UAB.