800 MHz Triple Resonance Observe (TXO) Detection CryoProbe

描述(由申请人提供)：我们要求获得资金，以获得800兆赫的冷冻探头配件。我们已经为一台新的800兆赫仪器争取到资金，这将缓解对高场仪器的漫长等待时间和机会不足。要求这种支持的调查组(Gerhard Wagner、James Chou、Christopher Walsh、Ellis Reherz和Anders Naar)可以在750 MHz及以上频率运行的高场仪器仅限于一个750 MHz光谱仪和半个900 MHz系统。尽管获得的机会有限，但这个小组在确定非常大的和具有挑战性的系统的结构和功能研究方面非常成功，这些系统包括膜蛋白、翻译起始因子复合体、非核糖体多肽合成酶、转录激活剂/辅激活剂复合体、T细胞蛋白复合体等等。许多新项目正在筹备中，但由于无法获得高场仪器，进展缓慢，而且获得750或900兆赫光谱仪的等待时间往往长达两个月。为了缓解这一问题，我们已经从哈佛内部竞争和其他来源获得资金购买了一台非屏蔽800 MHz光谱仪，我们还获得了资金来翻新哈佛/麻省理工学院磁共振中心的空间，以容纳新仪器。然而，可用的资金仅足以购买带有常规室温探头的光谱仪，而不足以购买低温探头。为了最大限度地利用这台新仪器的能量，我们申请资金购买一个冷冻探头附件和两个针对1H探测(TCI)优化的三重共振低温探头，这将是3D和4D分散NOESY实验的主力，以及13C/15N探测优化探头，它将用于有望突破研究超大型系统极限的新颖实验。

DESCRIPTION (provided by applicant): We request funding to acquire a 800 MHz cryoprobe accessory. We have secured funding for a new 800 MHz instrument that will alleviate the long waiting times for and insufficient access to high-field instrumentation. The high-field instruments operating at 750 MHz and above available to the group of investigators asking for this support (Gerhard Wagner, James Chou, Christopher Walsh, Ellis Reinherz and Anders Naar) are limited to one 750 MHz spectrometer and half of a 900 MHz system. Despite this limited access, this group was very successful in determining structures and performing functional studies of very large and challenging systems, such as membrane proteins, complexes of translation initiation factors, non-ribosomal peptide synthetases, transcriptional activator/coactivator complexes, T-cell protein complexes and many more. Numerous new projects are in the pipeline but progress is slowed down due to the lack of access to high field instruments, and waiting times are often two months to get access to the 750 or 900 MHz spectrometers. To alleviate this, we have obtained funds to purchase a non-shielded 800 MHz spectrometer from a Harvard internal competition, and other sources, and we have secured funds to renovate space at the Harvard/MIT Center for Magnetic Resonance to house the new instrument. However, the funds available are only sufficient to purchase the spectrometer with a conventional room temperature probe and are not enough to buy a cryogenic probe. To optimally use the power of this new instrument we ask for funds for a cryoprobe accessory with one cryoplatform and two triple-resonance cryoprobes that are optimized for 1H detection (TCI), which will be the work horse for 3D ad 4D dispersed NOESY experiments, and a 13C/15N detection optimized probe, which will be used for novel experiments that promise to push the limit for studying very large system.