Subcellular Localization of Neuronal Ion Channels

描述（由申请人提供）：本研究的目的是了解神经元跨膜蛋白极化靶向的分子机制。这笔赠款的具体目标将集中在三个主要层次的两极化目标：1。通过隔室特异性内吞作用和囊泡对接将蛋白质靶向质膜。2.将含有轴突或树突蛋白的囊泡运输到适当的极化区室。3.将轴突和树突蛋白分选到单独的囊泡中。这项研究的最终目标是了解这些靶向机制如何产生高度极化的蛋白质表面分布。 蛋白质靶向在许多生理过程中起着重要作用，如突触连接的建立和维持。此外，运输缺陷与许多具有重大临床意义的神经系统疾病有关，如阿尔茨海默氏病和亨廷顿氏病。 公共卫生相关性：在神经元中，许多跨膜蛋白特异性靶向轴突或树突区室。该资助的目标是了解介导这种靶向的分子机制。

DESCRIPTION (provided by applicant): The purpose of the studies proposed in this grant is to understand the molecular mechanisms underlying polarized targeting of neuronal transmembrane proteins. The specific aims of this grant will concentrate on three main levels of polarized targeting: 1. Targeting of proteins at the plasma membrane through compartment-specific endocytosis and vesicle docking. 2. Trafficking of vesicles containing either axonal or dendritic proteins to the appropriate polarized compartment. 3. Sorting of axonal and dendritic proteins into separate vesicles. The ultimate goal of this research is to understand how these targeting mechanisms produce surface distributions of proteins that are highly polarized. Relevance Protein targeting plays an important role in many physiological processes such as the establishment and maintenance of synaptic connections. In addition, defects in trafficking are associated with numerous neurological diseases of major clinical significance such as Alzheimer's and Huntington's disease. PUBLIC HEALTH RELEVANCE: In neurons many transmembrane proteins are targeted specifically to either the axonal or dendritic compartment. The goal of this grant is to understand the molecular mechanisms that mediate this targeting.