Testing Surgery Guided by Molecular Fluorescence Imaging

描述(由申请人提供)： 这份提案描述了一项为期5年的外科学术生涯发展培训计划。这位首席研究员在华盛顿大学完成了医学博士/博士课程，并在加州大学圣地亚哥分校(UCSD)完成了ACGME认可的耳鼻咽喉头颈外科住院医师培训项目。她还在加州大学圣迭戈分校完成了颅底外科博士后奖学金，现在，她将通过独特的跨部门专业知识和资源的整合，扩大她在临床医学和科学研究方面的综合培训。钱存训博士将指导首席研究员的科学发展。钱存训博士是细胞生物学和神经生物学领域公认的领导者，并以开发用于监测细胞内过程的彩色荧光染料而闻名世界。自以来，他一直是霍华德·休斯研究员，现任加州大学圣地亚哥分校药理学系教授，培养了许多博士后研究员和研究生。为了加强培训，该计划还将设立一个由内科科学家组成的咨询委员会，他们是各自领域的领导者，将提供科学和职业建议。研究将集中在分子荧光引导手术的动物模型的开发上。具体目标包括1)建立小动物荧光引导手术的成像系统，2)建立荧光引导手术的动物模型，为手术的准确性提供量化的客观标准。3)建立全身成像结合荧光图像引导手术的动物模型。尽管近年来CT、MRI和PET成像在癌症的非侵入性诊断方面取得了进展，但实体恶性肿瘤的主要治疗方式是手术切除。然而，肿瘤的手术切除仍然依赖于个体外科医生使用诸如肿瘤的位置、纹理、颜色、与周围结构的关系等不可量化的标准来区分肿瘤和正常组织的能力。这个项目的相关性是为手术医生提供一个实时可见的灵敏的分子标志物，以在术中区分肿瘤和非肿瘤组织，从而指导手术切除。

DESCRIPTION (provided by applicant): This proposal describes a 5 year training program for the development of academic career in Surgery. The principal investigator has completed a combined M.D./Ph.D. program at the Washington University and an ACGME accredited residency training program in Otolaryngology-Head and Neck Surgery at the University of California San Diego (UCSD). She has also completed a postdoctoral fellowship in Skull Base Surgery at UCSD and now, will expand her combined training in clinical medicine and scientific research through an unique integration of interdepartmental expertise and resources. Dr. Roger Tsien will mentor the principal investigator's scientific development. Dr. Tsien is a recognized leader in the field of cell biology and neurobiology and is world renowned for developing colorful fluorescent dyes for monitoring intracellular processes. He has been a Howard Hughes Investigator since 1989, is a Professor in the Dept of Pharmacology at UCSD and has trained numerous postdoctoral fellows and graduate students. To enhance the training, the program will also have an advisory committee of physician scientists who are leaders in their fields and who will provide scientific and career advice. Research will focus on the development of an animal model for molecular fluorescence guided surgery. The specific aims include 1) develop an imaging system for fluorescence guided surgery in small animals, 2) develop animal models for fluorescence-guided surgery, with quantitative objective criteria for accuracy of excision 3) develop animal models for whole body imaging coupled with fluorescence image guided surgery Despite recent technological advances in the noninvasive diagnosis of cancers including CT, MRI and PET imaging, the main treatment modality for solid malignant tumors is surgical excision. However, surgical excision of tumors remains dependent on the individual surgeon's ability to differentiate tumor from normal tissue using non-quantifiable criteria such as tumor location, texture, color, relationship to surrounding structures etc. The relevance of this project is to provide the operating surgeon with a sensitive molecular marker visualizable in real time to differentiate tumors from non-tumor tissue intraoperatively, thereby guiding surgical excision.