Validation

与附加到计划项目拨款中的大多数核心资源设施不同，这里提出的新验证核心不仅仅是一个独立的支持单元。该核心的目的是确保从主要项目中进行的实验中获得的信号是有效的，这是进行更深入研究，特别是临床研究之前的必要步骤。我们将通过几种方式验证所生产的探针和方法，包括a)通过使用和/或开发合适的体外结合亲和力测定来表征纳米材料靶向部分；B)通过荧光或放射性手段确定纳米材料在细胞中的选择性靶向性；C)相关动物模型的体外药代动力学研究；d)通过小动物成像进行体内药代动力学和药效学研究；e)与分子和比较病理生物学部门合作，提供所有与动物相关的服务，包括良好实验室规范（GLP）前毒性测定。加布里埃尔森和布雷顿)。我们将进一步将任何成像研究与传统的离体和组织学方法进行关联，并在实际和有用的情况下，尝试将基于核磁共振的成像结果与其他方式交叉关联，特别是基于放射性核素的成像和使用光学（荧光和生物发光）成像的成像。在这方面，验证核心将进行一定程度的自己的研究- -而不仅仅是提供日常服务- -但这种研究将始终与主要项目有关。因为验证对于任何成像研究都是至关重要的，所以这个核心与项目的整合是明确的。该核心的目标是使从事重大项目研究的科学家能够专注于他们工作中最复杂的方面，与核心紧密联系不仅可以获得日常服务，而且可以确保其结果的有效性。

Unlike most core resource facilities appended to program project grants, the new Validation Core proposed here is more than a detached support unit. The purpose of this core is to assure that the signal obtained from the experiments performed in the major projects is valid, a necessary step before deeper studies, in particular those bound for the clinic, can be undertaken. We will validate the probes and methods produced in several ways, including a) characterization of nanomaterial targeting moieties through use and/or development of suitable binding affinity assays in vitro; b) determination of selective targeting of nanomaterials in cellule through fluorescent or radioactive means; c) ex vivo pharmacokinetic studies in relevant animal models; d) in vivo pharmacokinetic and pharmacodynamic studies through small animal imaging and e) provision of all animalrelated services, including pre-Good Laboratory Practice (GLP) toxicity assays, in collaboration with the Department of Molecular and Comparative Pathobiology (Drs. Gabrielson and Brayton). We will further perform correlation of any imaging studies with traditional ex vivo and histological approaches and will also attempt to cross-correlate, when practical and useful, the MR-based imaging findings with other modalities, especially radionuclide-based and those that employ optical (fluorescence and bioluminescence) imaging. In that regard the validation core will perform a certain degree of its own research - rather than merely providing routine services - but that research will always be related to the major projects. Because validation is critical to any imaging study, the integration of this core to the projects is clear. The goal of this core is to enable the scientists performing the studies of the major projects to focus on the most complex aspects of their work, Interfacing closely with the core not only to procure routine services, but to assure the validity of their results.