STEM CELL PRODUCTION CORE: ADULT ANIMAL MARROW STEM CELLS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 间充质干细胞或骨髓基质细胞(MSCs)是来自骨髓的成体干细胞的一个子集。这些细胞具有医学和治疗价值，因为它们已被证明可分化为成骨细胞、脂肪细胞、软骨细胞、肌细胞、星形胶质细胞、少突胶质细胞和神经元。由于其固有的可塑性，这些细胞有可能用于治疗大量的遗传性疾病。Bunnell博士已经成功地确定了恒河猴骨髓间充质干细胞的扩增和特性要求。干细胞生产核心设施(SCPC)专注于非人类灵长类MSCs的产生、维持和分布。我们常规从恒河猴骨髓和脂肪组织标本中制备间充质干细胞。目前，我们有一个从100多只不同年龄的恒河猴中产生的骨髓间充质干细胞细胞系的库，这些细胞系已经准备好并准备分发。SCPC目前不仅对基因治疗计划有很大的影响，而且对TNPRC内的其他部门，如TNPRC内的比较病理学，以及杜兰健康科学中心和彭宁顿生物医学研究中心内的部门和中心以及全国其他研究实验室都有很大影响。 为合作研究项目生产恒河猴干细胞制剂。为此，我们计划使用在上一个资助期开发的技术来分离干细胞(MSCs、NSPC和ESCs)，以扩大从不同年龄(胎儿、新生儿和成人)和不同性别的动物分离的各种干细胞库。这些细胞将被用来生成大型细胞库，供合作科学家在内部或外部使用。我们已经创造了一个广泛的干细胞库，供科学界用于不同年龄的动物。 继续描述非人灵长类干细胞的特性。我们在这些研究中的目标是描绘不同干细胞群体在成熟和/或分化过程中发生的细胞和分子事件。该部门的科学家将继续在体外比较各种干细胞的生物学特性。将产生能够最大限度地促进MSCs及其后代分化和增殖的恒河猴干细胞特异性体外分化的检测方法。体外鉴定的一个焦点将是所描述的所有干细胞沿神经谱系的分化。为了完成克拉贝氏病的治疗，干细胞需要分化为少突胶质细胞。为实现这一目标，将作出重大努力，开发和应用差异化协议。在过去的一年里，我们与路易斯安那州立大学的研究人员合作，对长期酒精暴露并感染SIV的猕猴体内分离的MSCs的频率和生物学特性进行了表征，以评估对骨髓腔和干细胞组成的影响。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Mesenchymal stem cells or marrow stromal cells (MSCs) are a subset of adult stem cells from bone marrow. These cells are of medical and therapeutic interest because they have been shown to differentiate into osteoblasts, adipocytes, chondrocytes, myocytes, astrocytes, oligodendrocytes and neurons. Due to their inherent plasticity, these cells have the potential to be useful for the treatment of a large number of genetic diseases. Dr. Bunnell has successfully defined the requirements for the expansion and characterization of rhesus macaque MSCs. The Stem Cell Production Core Facility (SCPC) focuses on generation, maintenance and distribution of nonhuman primate MSCs. We routinely prepare MSCs from rhesus macaque bone marrow and adipose tissue samples. We presently have a bank pf MSC cell lines generated from over 100 rhesus macaques of varying age prepared and ready for distribution. The SCPC presently has a large impact not only on the Gene Therapy Program, but on other divisions such as Comparative Pathology within the TNPRC, but also Departments and Centers within the Tulane Health Sciences Center and the Pennington Biomedical Research Center and other research labs nationally. Produce rhesus macaque stem cell preparations for collaborative research projects. For this we plan to use the techniques developed over the last funding period to isolate stem cells (MSCs, NSPCs and ESCs) to expand the bank of various stem cell types isolated from animals of various ages (fetal, newborn and adults) and sexes of animals. The cells will be used to generate large cell banks for use internally or externally by collaborating scientists. We have generated an extensive bank of stem cells for use by the scientific community for various ages of animals. Continue the characterization of nonhuman primate stem cells. Our goal in these studies will be to delineate the cellular and molecular events that take place during maturation and/or differentiation of the various stem cell populations. Scientists in the division will continue to compare the biologic properties of the various stem cells in vitro. Assays mediating in vitro differentiation specific to rhesus stem cells that will maximize the differentiation and proliferation of MSCs and their progeny will be generated. One focus of the in vitro characterization will be the differentiation along neural lineages for all of the stem cells described. In order to accomplish the treatment of Krabbe's disease, stem cells will need to undergo differentiation into oligodendrocytes. A significant effort will be made towards the development and application of differentiation protocols to accomplish this goal. Over the past year, we have collaborated with investigators form LSU on the characterization of the frequency and biologic properties of MSCs isolated form rhesus macaques exposed to chronic alcohol and infected with SIV to assess the effects on the composition bone marrow cavity and stem cells.