Mouse Genetics Core

该中心的所有三个项目都需要来自加州大学洛杉矶分校的突变小鼠。老鼠遗传学的核心将驻留在 加州大学洛杉矶分校的SPF设施，将促进突变小鼠的回交、交配和传播 中心的所有三个项目都需要。该设施将维护这些品系，对它们进行基因分型，然后将它们发送出去 在需要时提供给参与实验室。该设施的一个重要作用将是转移突变 转化成稳定的遗传背景，然后可以重新测试并送到中心的实验室。一些人 将在项目1中提出的遗传筛查中进行测试的小鼠中，有一半不处于稳定、平衡的基因 背景。鼠标遗传核心将使用速度遗传和其他技术来转移选定的 突变成为稳定和明确的遗传背景。该设施还将负责 建议在可塑性筛查中发现基因条件突变的小鼠的分布。这个 该中心研究的整合完全依赖于遗传背景的一致性 老鼠进行了测试。由于表型，包括大脑表型，对遗传背景很敏感，因此 不可能整合中心不同实验室的研究结果，如果每个实验室都使用老鼠和 不同的遗传背景。因此，提供遗传背景一致的小鼠是至关重要的关键 小鼠遗传核心的功能。

All three projects in the Center require mutant mice derived at UCLA. The Mouse Genetics Core will reside in a SPF facility at UCLA and will facilitate the back-cross, mating and dissemination of the mutant mice required by all three Projects in the center. The facility will maintain the lines, genotype them and send them when needed to the participating laboratories. An important role for the facility will be to transfer mutations into stable genetic backgrounds that could then be retested and sent to the laboratories in the Center. Some of the mice that will be tested in the genetic screen proposed in Project 1 are not in stable, balanced genetic backgrounds. The Mouse Genetics Core will use speed congenics and other techniques to transfer selected mutations into stable and defined genetic backgrounds. The facility will also be responsible for the distribution of mice with conditional mutations of genes identified in the plasticity screens proposed. The integration of studies in the Center is completely dependent on the uniformity of the genetic background of the mice tested. Since phenotypes, including brain phenotypes, are sensitive to genetic background, it would be impossible to integrate findings from different laboratories in the Center, if each laboratory used mice with varying genetic background. Thus, providing mice of uniform genetic background is a crucial and key function of the Mouse Genetics Core.