Polyphenol-protein antioxidants in the Gl environment

超出提供的空间。我们的长期目标是了解食用植物性食品和饮料中的多酚对健康的影响。食用富含水果、蔬菜和植物衍生饮料的饮食可能会降低与氧化应激有关的疾病的风险，包括炎症性肠道疾病、心血管疾病和癌症。植物是营养抗氧化剂(维生素)的来源，但也可能是非营养抗氧化剂的来源。多酚是植物中最常见的一类次生产物，在体外也是有效的抗氧化剂。我们认为，被称为聚合物多酚的特定类别的多酚具有其他非营养型抗氧化剂所不具备的独特属性。聚合物多酚与蛋白质具有高亲和力，因此多酚-蛋白质复合体是胃肠道中的主要物种。我们推测，与蛋白质的结合改变了聚合物多酚的性质，如寿命和抗氧化活性，使它们特别适合于缓解炎症性肠病。我们假设，聚合物多酚蛋白加合物提供了一个持续的、高容量的活性池，定位于Gl道，因此能够直接减轻炎症性肠病部位的氧化损伤和炎症。植物多酚有数千种，分为几个不同的结构类别。对我们的假设进行体内测试是不可能的，因为目前还不清楚哪些化合物可能是最具生物活性的。我们建议进行体外研究，以考察10种不同的多酚及其蛋白质加合物在胃肠道条件下的持久性。然后，我们将检查这些多酚及其蛋白质加合物的抗氧化和抗炎活性。这将使我们能够确定在活体内最有可能缓解炎症性肠病的饮食多酚类型，并使我们能够在未来的提案中继续在动物身上进行试验。表演网站========================================Section End===========================================

EXCEED THE SPACE PROVIDED. Our long-term goal is to understand the health-related effects of consuming polyphenols found in plant- based foods and beverages. Eating a diet rich in fruits, vegetables, and plant-derived beverages may reduce the risk of diseases that have been linked to oxidative stress, including inflammatory bowel disease, cardiovascular disease and cancer. Plants are a source of nutritive antioxidarits (vitamins) but may also be a source of non-nutritive antioxidants. Polyphenols are the most common group of plant secondary products, and are effective antioxidants in vitro. We believe that the specific class of polyphenols known as the polymeric polyphenols have unique attributes not shared by other non-nutritive antioxidants. Polymeric polyphenols bind proteins with high affinity, so that polyphenol-protein complexes are the major species in the gastrointestinal tract. We speculate that binding to protein alters the propertiessuch as lifetime and antioxidant activitiesof polymeric polyphenols, making them uniquely suited to alleviating inflammatory bowel disease. We hypothesize that polymeric polyphenol- protein adducts provide a pool of persistant, high capacity activity that is localized to the Gl tract and is thus able to alleviate oxidative damage and inflammation directly at the site of inflammatory bowel disease. There are thousands of plant polyphenols, in several distinct structural classes. Conducting in vivo tests of our hypothesis would be impossible because it is not clear which compounds might be most bioactive. We propose in vitro studies to examine the persistence of ten different polyphenols and their protein adducts under gastrointestinal conditions. We will then examine the antioxidant and anti-inflammatory activities of these polyphenols and their protein adducts. This should allow us to identify types of dietary polyphenols that are most likely to alleviate inflammatory bowel disease in vivo, and position us to continue with testing in animals in a future proposal. PERFORMANCE SITE ========================================Section End===========================================