Animal Care Core

精确的代谢表型分析在很大程度上取决于动物受试者的健康状况。稳定的圈养和饲养以及微生物检测可最大限度地减少可能影响表型分析结果的外源性因素的影响。检疫和测试小鼠以确保可接受的健康状态所固有的严格性必须与加快新到达的小鼠的表型鉴定的需要相平衡。动物中心将提供最先进的饲养、医疗保健、检疫、检测和程序空间，用于评估提交给小鼠代谢表型中心（MMPC）的小鼠。动物护理中心已实施了一项加速检疫计划，该计划已在过去9年中生效。这成功地减少了检疫时间，并允许在动物处于检疫期间开始进行表型分析，从而允许在更年轻的年龄对动物进行研究，并最大限度地降低了与表型分析过程相关的成本。核心将：1）进口小鼠进行代谢表型分析，2）确定每个进口队列的微生物学特征，并向中心主任提供有关结果的解释和建议，3）为提交给中心的小鼠提供稳定的生物防护圈养、饲养和医疗保健，4）提供专用的生物安全房间，用于检疫、圈养和现场表型分析程序，5）根据要求进行形态表型分析，6）向中心客户提供结果和提交小鼠健康状况的建议。这些设施包括600 ft^的专用生物防护外壳和程序空间，配备有160笼通风架、NMR分析仪和8个代谢（热量测定）笼。根据过去5年的核心数量，预计核心每年进口802 +/- 215只小鼠。

Precise metabolic phenotyping depends significantly on the health status of animal subjects. Stable housing and husbandry and microbiological testing minimize the influence of exogenous factors that could impact phenotyping results. The rigors inherent to quarantine and testing mice to ensure acceptable health status must be balanced with the need to expedite phenotyping of newly arrived mice. The Animal Core will provide state-of-the-art husbandry, health care, quarantine, testing, and procedure space for assessing mice submitted to the Mouse Metabolic Phenotyping Center (MMPC). The Animal Care Core has implemented an expedited quarantine program that has been effect for the past 9 years. This has successfully decreased the quarantine time and allowed phenotyping to begin while animals are within quarantine, allowing animals to be studied at younger ages and minimizing costs associated with the phenotyping process. The Core will: 1) import mice for metabolic phenotyping, 2) determine the microbiological profile of each imported cohort and provide the Center Director with interpretation and advice about the results, 3) provide stable, biocontainment housing, husbandry and health care for mice submitted to the Center, 4) provide a dedicated, biosecure suite of room for quarantine, housing and on-site phenotyping procedures, 5) perform morphological phenotyping as requested, 6) provide to Center clients results and advice on the health status of submitted mice. The facilities include 600 ft^ of dedicated biocontainment housing and procedure space equipped with 160 cage ventilated rack, an NMR analyzer, and 8 metabolic (calorimetry) cages. The core is expected to import 802 +/- 215 mice per year, based on core volume during the previous 5 years.