Tissue and Keratinocyte Procurement

组织和角质形成细胞采购核心的作用是促进初级组织的获取 以及来自正常人类皮肤、患者样本和基因工程小鼠的角质形成细胞。 在基因工程小鼠中发现皮肤表型的研究人员需要确定 在人类疾病中也观察到类似的表型。此外，想要确定一个 特定的分子或途径参与皮肤生理需要来自基因工程的原代角质形成细胞 用于分析的小鼠。皮肤生物学家经常使用人类皮肤模型，包括皮肤器官培养和 人类皮肤移植，这需要持续采购正常的人类皮肤。为了促进这些 研究，核心B将提供专业知识、服务和培训，以获得这些主要组织和 相关的内部审查委员会批准。由于原代培养的角质形成细胞来自新鲜分离的人皮肤， CORE可以使用经济高效和统一的方法来分配这些组织。此外，核心B将建立 保存在皮肤病理学临床实验室的患者皮肤活检标本的组织库。当一个 皮肤或毛囊表型鉴定，相关石蜡包埋样本来自Core B组织库 将被识别并提供给核心A(皮肤组织学和特征)以进行切片和 组织病理学分析。核心B还将与核心C(干细胞和异种移植)协调，以获取 用于异种移植的正常人类皮肤。因此，核心B提供的独特服务确保了高效的 在动物和人类皮肤模型中初级组织的分布和促进翻译研究 疾病，特别是对于没有皮肤生物学经验或接触到患者标本的中心研究人员。 该中心的目标是：1)提供获取原代角质形成细胞的专业知识、服务和培训 培养，2)提供专业知识和服务，获取福尔马林固定的正常人皮肤切片，以及 通过建立人类皮肤疾病患者皮肤活检标本组织库，以及3)提供 新鲜分离的正常人皮肤，用于生理学和病理生理学研究。

The role of the Tissue and Keratinocyte Procurement Core is to facilitate the acquisition of primary tissue and keratinocytes from normal human skin, patient specimens, and genetically-engineered mice. Researchers who discover a skin phenotype in genetically-engineered mice need to identify whether a similar phenotype is observed in human disease. Additionally, researchers who want to determine if a particular molecule or pathway is involved in skin physiology require primary keratinocytes from geneticallyengineered mice for analysis. Skin biologists often use human skin models, including skin organ culture and human skin xenografts, which requires continuous procurement of normal human skin. To facilitate these studies, core B will provide expertise, service, and training in obtaining these primary tissues and the relevant IRB approvals. Since primary keratinocyte cultures are derived from freshly isolated human skin, the core can distribute these tissues using cost-effective and uniform methods. Additionally, Core B will establish a tissue bank from patient skin biopsy specimens stored in the dermatopathology clinical laboratory. When a skin or hair follicle phenotype is identified, relevant paraffin embedded samples from the Core B tissue bank will be identified and provided to Core A (Skin Histology and Characterization) for sectioning and histopathological analysis. Core B will also coordinate with Core C (Stem Cell and Xenograft) to procure normal human skin for xenografting. The unique services provided by core B therefore ensure the efficient distribution of primary tissues and facilitate translational research in animal and human models of skin disease, especially for Center researchers with no experience in skin biology or access to patient specimens. The Aims of the core are: 1) To provide expertise, service and training in obtaining primary keratinocyte cultures, 2) To provide expertise and service in obtaining formalin-fixed sections of normal human skin and human skin diseases by establishing a tissue bank of patient skin biopsy specimens, and 3) To provide freshly isolated normal human skin for physiologic and pathophysiologic studies.