Analytical Ultracentrifuge Shared Instrumentation

说明(由申请人提供)：本建议书用于购买分析性超离心机(Beckman Coulter型号XL-I型)。该仪器将提供在溶液条件下最先进的大分子行为表征的能力，可以揭示关于大分子构象、与大分子和小分子的相互作用、自缔合和样品异质性的信息。由于这些生物物理特性决定了生物系统的功能、抑制和/或调节的许多方面，因此研究这些特性的能力将显著增强俄亥俄州立大学NIH资助的大量研究项目的影响和广度。由于俄亥俄州立大学目前没有分析性超离心机，此次购买将使生物医学相关研究成为可能，这些研究目前由于访问有限和场外收费服务设施的高昂成本而无法实现。许多实验需要对样品制备、缓冲条件、运行时间等进行广泛的优化，但由于时间和费用的限制，这在异地进行是不可行的。拥有现场仪器将对氨基酰-tRNA合成酶编辑复合体的研究、艾滋病毒感染的关键病毒-宿主细胞相互作用、蛋白质失稳引起的疾病、基因调控的分子基础、组蛋白修饰的作用以及人类DNA聚合酶的研究产生重大影响。

DESCRIPTION (provided by applicant): This proposal is for the purchase of an analytical ultracentrifuge (Beckman Coulter model XL-I). This instrument will provide the capability for state-of-the-art characterization of macromolecular behavior under solution conditions, which can reveal information about macromolecular conformation, interactions with large and small molecules, self-association and sample heterogeneity. Since these biophysical properties determine many aspects of the function, inhibition and/or regulation of biological systems, the ability to study these properties will significantly enhance the impact and breadth of a large number of NIH-funded research programs at Ohio State University. As there is currently no analytical ultracentrifuge at Ohio State, this purchase will enable biomedically relevant studies that are currently not possible due to limited access and high cost of off-site fee-for-service facilities. Many experiments require extensive optimization of sample preparation, buffer conditions, run times, etc., which is not feasible to do off-site due to time constraints and expense. Having an on-site instrument will have a major impact on the investigation of aminoacyl-tRNA synthetase editing complexes, viral-host cell interactions critical for HIV infection, diseases caused by protein destabilization, the molecular basis of gene regulation, the role of histone modifications, and on studies of human DNA polymerases.