High Throughput Drug Screening Robot

描述（由申请人提供）：爱荷华州大学的教师从事研究和医疗保健的使命，以促进我们对疾病的理解和治疗。目前，爱荷华州大学拥有最先进的设施，专注于蛋白质组学，显微镜，流式细胞术和成像，但我们的使命发现新的分子来理解和治疗疾病将大大推进高通量分析工作站。该提案要求资金为大学内的单位致力于建立的新的高通量筛选（HTS）设施获得高通量检测工作站（药物发现机器人）。所要求的检测工作站将使研究人员能够优化和筛选小分子文库（ca. 100，000种化合物），以96孔板、384孔板和1536孔板的形式。该系统具有集成的平板/化合物存储模块，带有温控培养箱、完整的液体处理机器人和高速平板读取器，具有荧光、吸光度、发光和AlphaScreen”多种检测模式。该系统将采用定制设计，并通过单一软件套件进行控制，以提供最友好的用户体验，同时保持最高的筛选速度和精度。此外，该系统将允许研究人员创建新的肽库，这些肽库可以由使用该工作站的大学其他研究人员进行筛选。购买检测工作站的建议书为新计划的HTS设施奠定了仪器基础，该设施得到了大学，临床转化科学研究所以及卡弗医学院和药学院基础科学部门的支持，仪器和设施将被安置在那里。爱荷华州大学没有这样的仪器，在这个应用程序中，来自校园内许多单位的研究人员表达了他们对这种仪器的需求和支持，以进一步推进他们的NIH资助的研究计划，重点是新药发现，靶标验证，化合物库建设，以及开发新的分子工具来回答基本的科学问题。

DESCRIPTION (provided by applicant): The faculty at the University of Iowa is engaged in a mission of research and healthcare to advance our understanding and the treatment of diseases. Currently, the University of Iowa houses state-of-the-art facilities focused on proteomics, microscopy, flow cytometry and imaging, however our mission of finding novel molecules to understand and treat diseases would be greatly advanced by a High Throughput Assay Workstation. This proposal requests funds to obtain a High Throughput Assay Workstation (Drug Discovery Robot) for a new High Throughput Screening (HTS) facility that units within the university are committed to establishing. The requested Assay workstation will enable investigators to optimize and screen small molecule libraries (ca. 100,000 compounds) in formats of 96-, 384- and 1536-well plates. The system has an integrated plate/compound storage module with a temperature-controlled incubator, complete liquid handling robotics, and a high-speed plate reader with multiple detection modes for fluorescence, absorbance, luminescence, and AlphaScreen". The system will be custom engineered and controlled with a single software suite to allow the user-friendliest experience, while maintaining the highest speed and precision for screening. In addition, this system will allow investigators to create new peptide libraries that could be screened by other investigators at the university that use the workstation. This proposal for the purchase of the Assay Workstation forms the instrumental foundation for a newly planned HTS facility that has guaranteed support from the University, the Institute for Clinical Translational Science, and the basic science departments in the Carver College of Medicine and the College of Pharmacy, where the instrument and facility will be housed. No such instrumentation exists at the University of Iowa, and in this application, researchers from across many units on campus express their need and support for such an instrument to further their NIH-funded research programs, with focuses on new drug discovery, target validation, compound library construction, and the development of novel molecular tools to answer basic scientific questions.