Zeiss PALM MicroBeam IV Laser Capture System

描述（由申请人提供）：俄勒冈州州立大学的环境健康科学中心（EHSC）和基因组研究和生物计算中心（CGRB）的20名主要研究人员申请蔡司PALM MicroBeam IV激光捕获系统的资金。这种特殊的激光显微切割系统的关键和目前独特的功能是压力捕获技术，提供了非接触捕获新鲜组织。这种能力，这是不可能的其他激光捕获系统，将使我们能够执行自上而下 不稳定的翻译后修饰和单个活细胞分离的蛋白质组学。请求的PALM MicroBeam IV激光捕获系统在2010年2月8日至10日举行的为期三天的演示中表现出色，在此期间成功测试了该系统的几种新用途。这组研究人员主要由NIH资助，研究范围广泛的生物医学相关主题，从致癌作用和神经变性，到纳米颗粒和昼夜节律蛋白，再到细胞信号传导和组织中的其他基本功能。这种最先进的系统将提供一种新的工具，用于通过分析受影响组织的特定细胞群来确定疾病发展和对环境压力的反应所涉及的生化和分子机制。目前，非接触式激光捕获显微切割系统在俄勒冈州不存在，获得这项技术将支持大学范围内的计算和基因组生物学和健康老龄化研究的战略举措，以及与当地好撒玛利亚医院、分子探针/英杰公司、俄勒冈州大学和西部健康科学大学的健康研究中心的合作，并促进培养现代细胞生物学技术的研究生和博士后研究人员。来自大学的大力支持使EHSC和CGRB能够为研究提供最先进的技术，这两个单位寻求建立在这种合作的基础上，以充分支持所要求的激光捕获系统，并确保其有效的利用和维护。CGRB和EHSC之间类似的行政合作已经进行了五年，以成功地运营和维护蔡司 共聚焦显微镜，并提供方便地访问大图像文件，以用户从四个学院。拟议的蔡司PALM MicroBeam IV激光显微切割系统将由20个不同的实验室使用，支持25个NIH研究基金。三个是大型多研究者赠款，这是一个新的NCI计划项目赠款，一个新的NIEHS超级基金研究计划，以及最近更新的NIEHS资助的P30核心中心。 公共卫生豁免：为了了解某些疾病的发展和进展，提取小的组织样本进行分析变得很重要。激光捕获显微切割为科学家提供了从组织中去除少量受损细胞的能力，以确定与疾病有关的特定因素。

DESCRIPTION (provided by applicant): A group of 20 principal investigators associated with the Environmental Health Sciences Center (EHSC) and the Center for Genome Research and Biocomputing (CGRB) at Oregon State University request funds for a Zeiss PALM MicroBeam IV Laser Capture System. The critical and currently unique feature of this particular laser microdissection system is the pressure-catapulting technology that provides non-contact capture of fresh tissues. This capability, which is not possible by other laser capture systems, will enable us to perform top down proteomics of labile post-translational modifications and single live cell isolation. The requested PALM MicroBeam IV Laser Capture System performed exceedingly well during a three day demonstration held February 8-10, 2010, and several new uses for the system were successfully tested during this time. This group of researchers is funded, primarily by NIH, to investigate a wide range of biomedically-related topics, from carcinogenesis and neurodegeneration, to nanoparticles and circadian proteins, to other basic functions in cellular signaling and organization. This state-of-the-art system will provide a new tool for determining the biochemical and molecular mechanisms involved in the development of disease and response to environmental stressors through analysis of specific cellular populations from affected tissues. Currently, a non-contact laser capture microdissection system does not exist in the state of Oregon and access to this technology would support University-wide strategic initiatives in Computational and Genome Biology and Healthy Aging Research and collaborations with the Center for Health Research at the local Good Samaritan Hospital, Molecular Probes/Invitrogen, University of Oregon, and the Western University of Health Sciences, as well as promote the training of graduate students and postdoctoral researchers in modern cell biological techniques. Strong support from the University has enabled the EHSC and CGRB to provide state-of-the-art technology for research and the two units seek to build on this collaboration to fully support the requested laser capture system and ensure its efficient utilization and maintenance. A similar administrative collaboration between the CGRB and EHSC has been in place for five years to successfully operate and maintain a Zeiss confocal microscope and provide ready access to large image files to users from four colleges. The proposed Zeiss PALM MicroBeam IV laser microdissection system will be used by 20 different laboratories supporting 25 NIH research grants. Three are large multi-investigator grants, which are a new NCI Program Project grant, a new NIEHS Superfund Research Program, as well as the recently renewed NIEHS-funded P30 Core Center. PUBLIC HEALTH RELEVNANCE: To understand the development and progression of certain diseases, it has become important to extract small samples of tissue for analysis. Laser capture microdissection provides scientists with the capability to remove as little as a few damaged cells from the tissue in order to determine specific factors involved in the disease.