Vevo 2100

描述(由申请人提供)：这是一份重新提交的购买设备的申请，以支持为小动物提供最先进的共享心血管生理学资源(CPR)，包括一台高分辨率的数字超声仪(Vevo 2100)和可同时进行成像和血流动力学询问的有创血流动力学系统。拟议的资源将满足该机构对能够在单一地点进行复杂的心血管生理学评估的现代设施的迫切需求，并将现有的有限的成像资源转变为全面的单一地点的生理学资源。拟议的资源将升级现有的机械超声机，该超声机对心脏生理学来说不是最理想的，而是一台固态、电子、高分辨率的超声机。此外，配套的血流动力学系统的可用性将允许研究人员在小动物身上同时进行有创性血流动力学测量，这目前是不可行的。拟议资源将为医学系(心脏病)、儿科、麻醉学、公共卫生学院和生物医学工程系的5名主要用户和4名次要用户提供支助。所有主要用户都有NIH资助的合格项目，其中特别包括广泛的心血管生理学研究，这些研究目前是分片提供的或通过校外合作进行的。所有主要用户都可以通过已建立的和拟议的CPR基础设施使用该仪器和进行培训。机构资金和使用费将用于支持服务合同和其他关键升级。在过去四年中，这一战略已成功地在成像资源中实施，我们预计在拟议的CPR中继续这一安排不会有任何困难。虽然我们预计CPR的使用率会很高，但我们会为新用户保留20%的时间。现有的成像资源在约翰·霍普金斯大学的几个实验室小组中有教育、培训和生产力的记录。拟议中的CPR将是机构中唯一可用的心血管生理学设施，该机构拥有一支充满活力和高生产率的基础心血管研究人员团队。由于新的Vevo 2100包含重大的和具有科学意义的技术升级，而现有的Vevo 770即将缺乏部件和服务支持，因此所要求的仪器对于继续和推进这一研究势头至关重要。 公共卫生相关性：心血管疾病的小动物模型对于理解机制和开发新的治疗方法至关重要，然而，这项工作的一个基本组成部分是仔细和详细地评估心血管生理学，通常使用有创和非侵入性方法相结合。拟议的心血管生理学资源将为一大批高生产率的心血管研究人员提供便利、单一地点的设施，为全面的小动物生理学研究提供现代、复杂的设备。

DESCRIPTION (provided by applicant): This is a resubmission of an application to purchase equipment to support a state-of-the-art shared Cardiovascular Physiology Resource (CPR) for small animals consisting of a high resolution, digital ultrasound machine (Vevo 2100) and an invasive hemodynamic system that would allow simultaneous imaging and hemodynamic interrogation. The proposed resource would address a critical need in this institution for a modern facility capable of sophisticated cardiovascular physiology assessment located at a single site and would convert the existing, limited, imaging resource to a comprehensive, single-site physiology resource. The proposed resource would upgrade an existing mechanical ultrasound machine that is suboptimal for cardiac physiology with a solid-state, electronic, high resolution ultrasound machine. In addition, the availability of a companion hemodynamics system would allow researchers to perform simultaneous invasive hemodynamic measurements in small animals, not currently feasible. The proposed resource will support 5 major and 4 minor users from the Departments of Medicine (Cardiology), Pediatrics, Anesthesiology, School of Public Health and Biomedical Engineering. All major users have qualifying NIH-funded projects that specifically include extensive cardiovascular physiology studies that are currently available piece-meal or through extramural collaborations. All major users will have access to the instrument and training through an established and proposed CPR infrastructure. Institutional monies and usage charges will be used to support service contract and other critical upgrades. This strategy has been successfully implemented for the imaging resource over the last four years and we foresee no difficulty in continuing that arrangement for the proposed CPR. Although we anticipate a high use percentage for the CPR, we will reserve 20% time for new users. The existing imaging resource has a track record of education, training, and productivity with several lab groups at Johns Hopkins University. The proposed CPR will be the only available cardiovascular physiology facility in an institution with a vibrant and highly productive group of basic cardiovascular researchers. Since the new Vevo 2100 contains significant and scientifically meaningful technical upgrades and there is an impending lack of parts and service support for the existing Vevo 770, the requested instrumentation is critical to continue and advance the scope of this research momentum. Public Health Relevance: Small animal models of cardiovascular disease are critical to understanding mechanisms and developing new therapies, however, an essential component of this effort is careful and detailed evaluation of cardiovascular physiology usually performed using a combination of invasive and non-invasive methods. The proposed Cardiovascular Physiology Resource will provide modern, sophisticated equipment for comprehensive small animal physiology studies in a convenient, single-site facility for a large group of highly productive cardiovascular researchers.