Mosquito larval immunity and its impact on gut microbiota and malaria competence

描述（由申请人提供）：疟疾媒介能力在很大程度上取决于肠道微环境中蚊子肠道微生物群、疟原虫和宿主免疫之间的生态相互作用。蚊子

DESCRIPTION (provided by applicant): Malaria vector competence is largely determined by ecological interactions among mosquito gut microbiota, Plasmodium, and host immunity in the context of gut microenvironment. Mosquito immunity studies have been mainly focusing on the adults. Larva-pathogen interaction is an unignorable driving force in shaping mosquito immune system during the evolution. However, little is known about larva immune structure and its interaction with gut microbial flora. In this project we propose to use larvicidal Bacillus sphaericus to study mosquito larval immune response, and characterize larval immune architecture by using microarray based global expression profiling. The impact of larval immune activation on the dynamics of gut microbiota in both larvae and adults will be assessed by profiling gut bacterial community via high throughput pyrosequencing bacterial 16S rDNA marker. The influence of these parameters on the malaria susceptibility will also be evaluated. The project will generate data towards a better understanding of larval immunity and its impact on mosquito gut ecosystem.