GABA AND GLUCOCORTICOID REG OF CRH NEURONS AND EXTINCTION OF FEAR

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 我们开始了我们的研究，观察在含有GABAγ2的细胞中缺乏CRH表达的小鼠的内分泌学、分子和行为特征。我们现在正在使用原位杂交技术研究包括杏仁核、BNST、PVN和丘脑在内的多种结构。此外，我们正在优化荧光原位杂交探针，以在细胞水平上表征CRH和GABA Gamma 2的共定位。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. We began our study looking at endocrinological, molecular and behavioral characterization of mice lacking CRH expression in GABA gamma 2 containing cells. We are now looking at multiple structures including the amygdala, BNST, PVN and thalamus using in situ hybridization. Additionally, we are optimizing Fluorescent in situ hybridization probes to characterize colocalization of CRH and GABA gamma 2 at the cellular level.