Retroviral Transduction Core

该项目的许多实验方案依赖于原代或培养细胞的逆转录病毒转导。由于逆转录病毒制备和转导技术需要高度的技术专长，因此在中央设施实施这些技术将极大地促进所有项目的进展。一个用于逆转录病毒制备和转导的中央设施将为该集团提供多种优势。这些包括：1)制备高滴度逆转录病毒上清的统一程序，2)高滴度逆转录病毒上清的定期可获得性，3)逆转录病毒上清的定期监测，以确保它们不含支原体和不含复制能力逆转录病毒，以及4)胎肝和骨髓造血干细胞转导方面的专业知识，由于研究计划的协作性，高滴度逆转录病毒上清液和转导程序的可用性，以及方案和试剂的标准化，将对该计划中的每个人都有很大的好处。逆转录病毒核心将被安置在Pear博士的研究空间中。核心将购买和维护这些程序所需的设备。此外，核心还将批量购买消耗品，以确保材料的标准化和批量购买折扣。

Many of the experimental protocols for the projects in this program rely on retroviral transduction of primary or cultured cells. Because the techniques of retroviral preparation and transduction require a high degree of technical expertise, a central facility where these techniques are performed would significantly enhance the progress of all projects. A central facility for retroviral preparation and transduction would offer multiple advantages to the group. These include: 1) uniform procedures for preparing high titer retroviral supernatants, 2) regular availability of high titer retroviral supernatants, 3) periodic monitoring of retroviral supernatants to insure that they are mycoplasma-free and do not contain replication competent retrovirus, and 4) expertise in transduction of fetal liver and bone marrow hematopoietic stem cells, Because of the collaborative nature of the research program, availability of high titer retroviral supernatants and procedures for transduction, together with standardization of protocols and reagents would be of great benefit to everyone in the program. The retroviral core will be housed in Dr. Pear's research space. The Core will purchase and maintain equipment necessary for these procedures. Additionally, consumables will also be purchased in bulk by the Core to ensure standardization of materials and bulk purchase discounts.