Data Collection

在本P01中，我们将汇总AA中4项最大的乳腺癌研究的数据、样本和专业知识 妇女，卡罗莱纳乳腺癌研究（CBCS），妇女健康研究圈（WCHS），黑人 妇女健康研究（BWHS）和多种族队列（MEC）。我们会继续累积病例， 在CBCS和WCHS中分别招募了1000名患有乳腺癌的AA女性和1000名病例， 确定BWHS和MEC中的其他病例，最终样本量超过5，500例， 5，500个DNA对照，为进行亚型分析提供了足够的能力。重要的是，我们将收集 来自WCHS、CBCS和BWHS参与者的肿瘤组织块，并与核心C合作，使用 免疫组化（IHC）鉴定乳腺癌亚型。这将使我们能够追查几个 关于AA妇女早期侵袭性乳腺癌病因的假设， 独立但相互作用的项目。该计划中的4个科学项目的成功取决于 成功的活动，这一拟议的核心，积累和采访更多的情况下，并检索肿瘤 来自CBCS、WCHS和BWHS参与者接受手术的医院的组织块。我们 我们每个机构都有这些努力的经验，CBCS和WCHS在以下方面的经验 块收集将通知我们通过核心B进行的BW HS工作。 该核心的具体目标是：1）进行数据收集（案件查明，与 医生和病人，采访，DNA收集）在WCHS和CBCS。收集唾液 来自BWHS中新乳腺癌病例的样本;以及2）请求、收集和处理组织块， WCHS、CBCS和BWHS的病理学报告，对所有研究中心采用统一的系统方法。 该核心对于增加可用于所有四个项目的乳腺癌病例数量至关重要 这个POI，并以协调的方式收集肿瘤块，以便我们可以表征乳腺癌 癌症亚型，并检查早期侵袭性乳腺癌的遗传和非遗传风险因素。

In this P01, we will pool data, samples and expertise from 4 of the largest studies of breast cancer in AA women, the Carolina Breast Cancer Study (CBCS), the Women's Circle of Health Study (WCHS), the Black Women's Health Study (BWHS), and the Multi-Ethnic Cohort (MEC). We will continue case accruals, enrolling an additional 1000 AA women with breast cancer in CBCS and 1000 cases in WCHS and ascertainment of additional cases in BWHS and MEC, for a final sample size of more than 5,500 cases and 5,500 controls with DNA, providing sufficient power to conduct subtype analyses. Importantly, we will collect tumor tissue blocks from participants in the WCHS, CBCS and BWHS and, in collaboration with Core C, use immunohistochemistry (IHC) to identify breast cancer subtypes. This will allow us to pursue several hypotheses regarding the etiology of early aggressive breast cancer in AA women in the context of 4 independent, but interacting, projects. The success of the 4 scientific projects in this Program relies upon the successful activities of this proposed Core, to accrue and interview additional cases and to retrieve the tumor tissue blocks from the hospitals where participants in CBCS, WCHS and BWHS received their surgery. We have experience in these endeavors at each of our institutions, and the experiences of CBCS and WCHS in block collection will inform our efforts for BWHS through Core B. The specific aims of this Core are to: 1) Conduct data collection (case ascertainment, contact with physicians and patients, interviews, DNA collection) in the WCHS and CBCS. Conduct collection of saliva samples from new breast cancer cases in the BWHS; and 2) Request, collect, and process tissue blocks and pathology reports for WCHS, CBCS and BWHS, using a unified systematic approach for all sites. This Core is essential for increasing the number of breast cancer cases available for use in all four projects of this POI, and for collection of tumor blocks in a coordinated fashion so that we can characteristic breast cancer subtypes and examine genetic and non-genetic risk factors for early, aggressive breast cancer.